Biochemistry
9th Edition
ISBN: 9781305961135
Author: Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher: Cengage Learning
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Chapter 13, Problem 5RE
RECALL What is the importance of methylation in the activity of restriction endonucleases?
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Chapter 13 Solutions
Biochemistry
Ch. 13 - RECALL What advantages does fluorescent labeling...Ch. 13 - RECALL What methods are used to visualize...Ch. 13 - REFLECT AND APPLY When proteins are separated...Ch. 13 - RECALL How does the use of restriction...Ch. 13 - RECALL What is the importance of methylation in...Ch. 13 - RECALL Why do restriction endonucleases not...Ch. 13 - Prob. 7RECh. 13 - Prob. 8RECh. 13 - RECALL What do the following have in common? MOM;...Ch. 13 - RECALL Give three examples of DNA palindromes.
Ch. 13 - RECALL What are three differences between the...Ch. 13 - RECALL What are sticky ends? What is their...Ch. 13 - RECALL What would be an advantage of using HaeIII...Ch. 13 - RECALL Describe the cloning of DNA.Ch. 13 - RECALL What vectors can be used for cloning?Ch. 13 - RECALL Describe the method you would use to test...Ch. 13 - RECALL What is blue/white screening? What is the...Ch. 13 - Prob. 18RECh. 13 - Prob. 19RECh. 13 - Prob. 20RECh. 13 - Prob. 21RECh. 13 - Prob. 22RECh. 13 - Prob. 23RECh. 13 - REFLECT AND APPLY What are the requirements for an...Ch. 13 - Prob. 25RECh. 13 - Prob. 26RECh. 13 - REFLECT AND APPLY The genes for both the a- and...Ch. 13 - REFLECT AND APPLY Outline the methods you would...Ch. 13 - Prob. 29RECh. 13 - Prob. 30RECh. 13 - Prob. 31RECh. 13 - Prob. 32RECh. 13 - RECALL Why is temperature control so important in...Ch. 13 - RECALL Why is the use of temperature-stable DNA...Ch. 13 - RECALL What are the criteria for good primers in a...Ch. 13 - REFLECT AND APPLY What difficulties arise in the...Ch. 13 - REFLECT AND APPLY Each of the following pairs of...Ch. 13 - RECALL What is qPCR?Ch. 13 - Prob. 39RECh. 13 - REFLECT AND APPLY Suppose that you are a...Ch. 13 - REFLECT AND APPLY Why is DNA evidence more useful...Ch. 13 - REFLECT AND APPLY Give the DNA sequence for the...Ch. 13 - Prob. 43RECh. 13 - Prob. 44RECh. 13 - Prob. 45RECh. 13 - Prob. 46RECh. 13 - Prob. 47RECh. 13 - RECALL Has proteomic analysis been done on...Ch. 13 - Prob. 49RECh. 13 - Prob. 50RECh. 13 - Prob. 51RECh. 13 - RECALL What are the key differences between DNA...
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- RECALL List several ways in which RNA is processed after transcription.arrow_forwardREFLECT AND APPLY Describe the recognition process by which the tRNA for N-formylmethionine interacts with the portion of mRNA that specifies the start of transcription.arrow_forwardREFLECT AND APPLY Why is a short RNA primer needed for replication?arrow_forward
- REFLECT AND APPLY How can breakdown in DNA repair play a role in the development of human cancers?arrow_forwardREFLECT AND APPLY List three mechanisms that relax the twisting stress in helical DNA molecules.arrow_forwardRECALL What would be an advantage of using HaeIII for a cloning experiment? What would be a disadvantage?arrow_forward
- REFLECT AND APPLY Diagram a section of DNA being transcribed. Give the various names for the two strands of DNA.arrow_forwardRECALL Do DNA-polymerase enzymes also function as exonucleases?arrow_forwardRECALL What are the key differences between DNA microarrays and protein microarrays, and how they are used in research?arrow_forward
- RECALL Distinguish between rho-dependent termination and intrinsic termination.arrow_forwardREFLECT AND APPLY Why is it more important for DNA to be replicated accurately than transcribed accurately?arrow_forwardRECALL Compare and contrast the properties of the enzymes DNA polymerase I and polymerase III from E. coli.arrow_forward
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