Biochemistry
Biochemistry
9th Edition
ISBN: 9781305961135
Author: Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher: Cengage Learning
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Chapter 13, Problem 1RE

RECALL What advantages does fluorescent labeling offer over radioactive methods of labeling DNA?

Expert Solution & Answer
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Interpretation Introduction

Interpretation:

The advantages of fluorescent labeling over radioactive labeling of DNA is to be determined.

Concept introduction:

Two methods are used to detect or visualize DNA fragments.

Radioactive labeling: labeling of DNA molecule with radioactive isotopes of phosphorus (P32) and sulfur (S-35).

Florescence: labeling of DNA molecule with fluorescent molecules of ethidium bromide.

Answer to Problem 1RE

Solution: In fluorescent labeling of a DNA molecule, fluorescent molecules of ethidium bromide are used. This fluorescent tag is safe, can be decomposed easily, and no special license for its use is needed.

Explanation of Solution

Two methods are used to detect or visualize DNA fragments. In radioactive labeling of DNA molecule, radioactive isotopes of phosphorus (P32) and sulfur (S-35) are used. In fluorescent labeling of DNA molecule, fluorescent molecules of ethidium bromide are used.

Since in radioactive labeling, radioactive molecule is used, which is unsafe, special license is needed to carry out the experiment. Also, special techniques are required that can be used to decompose the radioactive waste. But all these things are not needed in the case of fluorescent labeling.

Thus, fluorescent tag is safe, can be decomposed easily, and no special license is needed for its use.

Conclusion

The fluorescent tag is safe, can be decomposed easily, and no special license is needed for its use.

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Chapter 13 Solutions

Biochemistry

Ch. 13 - RECALL What are three differences between the...Ch. 13 - RECALL What are sticky ends? What is their...Ch. 13 - RECALL What would be an advantage of using HaeIII...Ch. 13 - RECALL Describe the cloning of DNA.Ch. 13 - RECALL What vectors can be used for cloning?Ch. 13 - RECALL Describe the method you would use to test...Ch. 13 - RECALL What is blue/white screening? What is the...Ch. 13 - Prob. 18RECh. 13 - Prob. 19RECh. 13 - Prob. 20RECh. 13 - Prob. 21RECh. 13 - Prob. 22RECh. 13 - Prob. 23RECh. 13 - REFLECT AND APPLY What are the requirements for an...Ch. 13 - Prob. 25RECh. 13 - Prob. 26RECh. 13 - REFLECT AND APPLY The genes for both the a- and...Ch. 13 - REFLECT AND APPLY Outline the methods you would...Ch. 13 - Prob. 29RECh. 13 - Prob. 30RECh. 13 - Prob. 31RECh. 13 - Prob. 32RECh. 13 - RECALL Why is temperature control so important in...Ch. 13 - RECALL Why is the use of temperature-stable DNA...Ch. 13 - RECALL What are the criteria for good primers in a...Ch. 13 - REFLECT AND APPLY What difficulties arise in the...Ch. 13 - REFLECT AND APPLY Each of the following pairs of...Ch. 13 - RECALL What is qPCR?Ch. 13 - Prob. 39RECh. 13 - REFLECT AND APPLY Suppose that you are a...Ch. 13 - REFLECT AND APPLY Why is DNA evidence more useful...Ch. 13 - REFLECT AND APPLY Give the DNA sequence for the...Ch. 13 - Prob. 43RECh. 13 - Prob. 44RECh. 13 - Prob. 45RECh. 13 - Prob. 46RECh. 13 - Prob. 47RECh. 13 - RECALL Has proteomic analysis been done on...Ch. 13 - Prob. 49RECh. 13 - Prob. 50RECh. 13 - Prob. 51RECh. 13 - RECALL What are the key differences between DNA...
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