Anatomy & Physiology
1st Edition
ISBN: 9781938168130
Author: Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble
Publisher: OpenStax College
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Textbook Question
Chapter 13, Problem 16RQ
Which non-nervous tissue develops from the neuroectoderm?
- respiratory mucosa
- vertebral bone
- digestive lining
- craniofacial bone
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 13 Solutions
Anatomy & Physiology
Ch. 13 - Watch this animation...Ch. 13 - Watch this video...Ch. 13 - Watch this video...Ch. 13 - Watch this video...Ch. 13 - Watch this video...Ch. 13 - Compared with the nearest evolutionary relative,...Ch. 13 - Watch this animation...Ch. 13 - Watch this video...Ch. 13 - Watch this animation...Ch. 13 - Figure 13.20 If you zoom in on the DRG, you can...
Ch. 13 - Figure 13.22 To what structures in a skeletal...Ch. 13 - Visit this site...Ch. 13 - Aside from the nervous system, which other organ...Ch. 13 - Which primary vesicle of the embryonic nervous...Ch. 13 - Which adult structure(s) arises from the...Ch. 13 - Which non-nervous tissue develops from the...Ch. 13 - Which structure is associated with the embryologic...Ch. 13 - Which lobe of the cerebral cortex is responsible...Ch. 13 - What region of the diencephalon coordinates...Ch. 13 - What level of the brain stem is the major input to...Ch. 13 - What region of the spinal cord contains motor...Ch. 13 - Brodmanns areas map different regions of the...Ch. 13 - What blood vessel enters the cranium to supply the...Ch. 13 - Which layer of the meninges surrounds and supports...Ch. 13 - What type of glial cell is responsible for...Ch. 13 - Which portion of the ventricular system is found...Ch. 13 - What condition causes a stroke? inflammation of...Ch. 13 - What type of ganglion contains neurons that...Ch. 13 - Which ganglion is responsible for cutaneous...Ch. 13 - What is the name for a bundle of axons within a...Ch. 13 - Which cranial nerve does not control functions in...Ch. 13 - Which of these structures is not under direct...Ch. 13 - Studying the embryonic development of the nervous...Ch. 13 - What happens in development that suggests that...Ch. 13 - Damage to specific regions of the cerebral cortex,...Ch. 13 - Why do the anatomical inputs to the cerebellum...Ch. 13 - Why can the circle of Willis maintain perfusion of...Ch. 13 - Meningitis is an inflammation of the meninges that...Ch. 13 - Why are ganglia and nerves not surrounded by...Ch. 13 - Testing for neurological function involves a...
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forward
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