Brock Biology of Microorganisms (14th Edition)
14th Edition
ISBN: 9780321897398
Author: Michael T. Madigan, John M. Martinko, Kelly S. Bender, Daniel H. Buckley, David A. Stahl, Thomas Brock
Publisher: PEARSON
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Chapter 11.15, Problem 2MQ
Summary Introduction
The process of isolating potential useful genes from the environment is termed as gene mining. In this process, the isolation of the genes is carried out without initially culturing the organisms that carry them. In the gene mining process, the DNA or RNA is directly isolated from the environmental samples and then cloned into the suitable vectors in order to construct a metagenomic library.
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The Snapdragon is a popular garden flower that comes in a variety of colours, including red, yellow, and orange. The genotypes and associated phenotypes for some of these flowers are as follows:
aabb: yellow
AABB, AABb, AaBb, and AaBB: red
AAbb and Aabb: orange
aaBB: yellow
aaBb: ?
Based on this information, what would the phenotype of a Snapdragon with the genotype aaBb be and why?
Question 21 options:
orange because A is epistatic to B
yellow because A is epistatic to B
red because B is epistatic to A
orange because B is epistatic to A
red because A is epistatic to B
yellow because B is epistatic to A
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
Chapter 11 Solutions
Brock Biology of Microorganisms (14th Edition)
Ch. 11.1 - Prob. 1MQCh. 11.1 - Prob. 2MQCh. 11.2 - Prob. 1MQCh. 11.2 - Prob. 2MQCh. 11.3 - Why is a primer needed at each end of the DNA...Ch. 11.3 - MINIQUIZ
• From which organisms are thermostable...Ch. 11.3 - How does RT-PCR differ from traditional PCR?Ch. 11.4 - What is the purpose of molecular cloning?Ch. 11.4 - Prob. 2MQCh. 11.4 - Prob. 3MQ
Ch. 11.5 - Prob. 1MQCh. 11.5 - How can site-directed mutagenesis be useful to...Ch. 11.5 - What are knockout mutations?Ch. 11.6 - Prob. 1MQCh. 11.6 - Prob. 2MQCh. 11.7 - Prob. 1MQCh. 11.7 - Prob. 2MQCh. 11.7 - Prob. 3MQCh. 11.8 - Prob. 1MQCh. 11.8 - Prob. 2MQCh. 11.9 - MINIQUIZ
• Describe the components needed for an...Ch. 11.9 - Prob. 2MQCh. 11.10 - Prob. 1MQCh. 11.10 - Prob. 2MQCh. 11.10 - Prob. 3MQCh. 11.11 - What major advantage does cloning mammalian genes...Ch. 11.11 - Prob. 2MQCh. 11.12 - Prob. 1MQCh. 11.12 - Prob. 2MQCh. 11.12 - Prob. 3MQCh. 11.13 - Prob. 1MQCh. 11.13 - Give an example of a genetically modified plant...Ch. 11.13 - How have transgenic salmon been engineered to...Ch. 11.14 - Explain why recombinant vaccines might be safer...Ch. 11.14 - Prob. 2MQCh. 11.15 - Explain why metagenomic cloning gives large...Ch. 11.15 - Prob. 2MQCh. 11.16 - Prob. 1MQCh. 11.16 - Prob. 2MQCh. 11.17 - What are biobricks?Ch. 11.17 - How was Escherichia coli modified to produce a...Ch. 11 - Prob. 1RQCh. 11 - Prob. 2RQCh. 11 - Describe the basic principles of gene...Ch. 11 - Prob. 4RQCh. 11 - Prob. 5RQCh. 11 - Prob. 6RQCh. 11 - Prob. 7RQCh. 11 - REVIEW QUESTIONS
8. What is a reporter gene?...Ch. 11 - Prob. 9RQCh. 11 - Prob. 10RQCh. 11 - Prob. 11RQCh. 11 - Prob. 12RQCh. 11 - Prob. 13RQCh. 11 - Prob. 14RQCh. 11 - Prob. 15RQCh. 11 - Prob. 16RQCh. 11 - Prob. 17RQCh. 11 - What is the Ti plasmid and how has it been of use...Ch. 11 - What is a subunit vaccine and why are subunit...Ch. 11 - How has metagenomics been used to find novel...Ch. 11 - Prob. 21RQCh. 11 - Prob. 22RQCh. 11 - Prob. 1AQCh. 11 - Suppose you have just determined the DNA base...Ch. 11 - Prob. 3AQCh. 11 - Prob. 4AQ
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