Brock Biology of Microorganisms (14th Edition)
14th Edition
ISBN: 9780321897398
Author: Michael T. Madigan, John M. Martinko, Kelly S. Bender, Daniel H. Buckley, David A. Stahl, Thomas Brock
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 11.11, Problem 1MQ
- What major advantage does cloning mammalian genes from mRNA or using synthetic genes have over PCR amplification and cloning of the native gene?
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
What would be your experimental strategy and the lab materials required to clone the complementary human gene using a mammalian gene in conjunction with PCR?
You just graduated from college and started working at a biotech startup called Scrofabulous. Your
first job assignment is to clone the pig gene for the hormone prolactin. Assume that the pig gene
for prolactin has not yet been isolated, sequenced, or mapped; What would be the most useful and
economical first step to go about identifying and cloning the pig gene for prolactin?
use the amino acid sequence of mouse prolactin to design a pair of degenerate oligonucleotide PCR primers
to PCR-amplify the pig prolactin gene.
RNAseq the pituitary gland of the pig, the most abundant gene is likely to to be prolactin
Conduct a proteome search for peptides that match parts of mouse prolactin protein
Sequence the pig genome, then translate the genome to find the gene predicted to encode for prolactin
Crystalize the mouse prolactin protein and use Google's DeepMind Al to find the closest amino acid
sequence in the pig proteome
What is the purpose of molecular cloning?
Chapter 11 Solutions
Brock Biology of Microorganisms (14th Edition)
Ch. 11.1 - Prob. 1MQCh. 11.1 - Prob. 2MQCh. 11.2 - Prob. 1MQCh. 11.2 - Prob. 2MQCh. 11.3 - Why is a primer needed at each end of the DNA...Ch. 11.3 - MINIQUIZ
• From which organisms are thermostable...Ch. 11.3 - How does RT-PCR differ from traditional PCR?Ch. 11.4 - What is the purpose of molecular cloning?Ch. 11.4 - Prob. 2MQCh. 11.4 - Prob. 3MQ
Ch. 11.5 - Prob. 1MQCh. 11.5 - How can site-directed mutagenesis be useful to...Ch. 11.5 - What are knockout mutations?Ch. 11.6 - Prob. 1MQCh. 11.6 - Prob. 2MQCh. 11.7 - Prob. 1MQCh. 11.7 - Prob. 2MQCh. 11.7 - Prob. 3MQCh. 11.8 - Prob. 1MQCh. 11.8 - Prob. 2MQCh. 11.9 - MINIQUIZ
• Describe the components needed for an...Ch. 11.9 - Prob. 2MQCh. 11.10 - Prob. 1MQCh. 11.10 - Prob. 2MQCh. 11.10 - Prob. 3MQCh. 11.11 - What major advantage does cloning mammalian genes...Ch. 11.11 - Prob. 2MQCh. 11.12 - Prob. 1MQCh. 11.12 - Prob. 2MQCh. 11.12 - Prob. 3MQCh. 11.13 - Prob. 1MQCh. 11.13 - Give an example of a genetically modified plant...Ch. 11.13 - How have transgenic salmon been engineered to...Ch. 11.14 - Explain why recombinant vaccines might be safer...Ch. 11.14 - Prob. 2MQCh. 11.15 - Explain why metagenomic cloning gives large...Ch. 11.15 - Prob. 2MQCh. 11.16 - Prob. 1MQCh. 11.16 - Prob. 2MQCh. 11.17 - What are biobricks?Ch. 11.17 - How was Escherichia coli modified to produce a...Ch. 11 - Prob. 1RQCh. 11 - Prob. 2RQCh. 11 - Describe the basic principles of gene...Ch. 11 - Prob. 4RQCh. 11 - Prob. 5RQCh. 11 - Prob. 6RQCh. 11 - Prob. 7RQCh. 11 - REVIEW QUESTIONS
8. What is a reporter gene?...Ch. 11 - Prob. 9RQCh. 11 - Prob. 10RQCh. 11 - Prob. 11RQCh. 11 - Prob. 12RQCh. 11 - Prob. 13RQCh. 11 - Prob. 14RQCh. 11 - Prob. 15RQCh. 11 - Prob. 16RQCh. 11 - Prob. 17RQCh. 11 - What is the Ti plasmid and how has it been of use...Ch. 11 - What is a subunit vaccine and why are subunit...Ch. 11 - How has metagenomics been used to find novel...Ch. 11 - Prob. 21RQCh. 11 - Prob. 22RQCh. 11 - Prob. 1AQCh. 11 - Suppose you have just determined the DNA base...Ch. 11 - Prob. 3AQCh. 11 - Prob. 4AQ
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Unneeded genes in an adult animal cell are permanently inactivated,making it impossible for most specialized cells to turn into any othercell type. How does this arrangement save energy inside a cell? Whydoes the ability to clone an adult mammal depend on techniques forreactivating these “dormant” genes?arrow_forwardSite directed mutagenesis is used to: 1.determine the critical base per sequences in a Genome 2. Create genomic libraries 3. Determine the critical amino acids in a protein that allowed to function 4. Amplify dna 5. sequence dnaarrow_forwardWhat is reproductive cloning? How is it done? (Hint: review the discussion on crispr)arrow_forward
- What is the advantage of using stem cells for genetherapy or gene editing?arrow_forwardWhat is the difference between cloning and genetic engineering? What is CRISPR and how does it work What is recombinant DNA? What is a plasmid What is a PCR and what does it do? List an ethical consideration when it comes to cloning or genetic engineering.arrow_forwardThere are a range of ethical issues associated with cloning. However, many of these are not applied to cloning plants. Explain why the idea of cloning an entire plant is generally accepted while cloning an entire human is not.arrow_forward
- For what reasons would gene therapy be more effective performed onembryos rather than fully developed animals?arrow_forwardWhat are the advantages of using organisms such as bacteria and yeast as cloning hosts? What safety considerations must be made when choosing a cloning host to make proteins for human use?arrow_forwardWhat is exome sequencing ? Why it is important ?arrow_forward
- Discuss three important advances that have resulted from gene cloning.arrow_forwardWhy is genome editing by CRISPR-Cas advantageous over traditionalmethods for creating knockout or transgenic animals?Explain your answers.arrow_forwardSome people have argued that editing the genomes of human embryos is ethically defensible as long as the embryos are not allowed to develop past an early stage and thus will never result in the birth of genetically modified humans. Others have argued that no genome editing should be carried out on human embryos. Present arguments for and against using genome editing on human embryos.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage Learning
Human Heredity: Principles and Issues (MindTap Co...
Biology
ISBN:9781305251052
Author:Michael Cummings
Publisher:Cengage Learning
Molecular Techniques: Basic Concepts; Author: Dr. A's Clinical Lab Videos;https://www.youtube.com/watch?v=7HFHZy8h6z0;License: Standard Youtube License