Brock Biology of Microorganisms (14th Edition)
14th Edition
ISBN: 9780321897398
Author: Michael T. Madigan, John M. Martinko, Kelly S. Bender, Daniel H. Buckley, David A. Stahl, Thomas Brock
Publisher: PEARSON
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Chapter 11.10, Problem 2MQ
Summary Introduction
The artificial chromosomes are the synthetic chromosomes that contain the DNA fragments integrated into the host chromosome. The DNA cloned vectors are sufficient for making gene libraries for sequencing the prokaryotic genomes. They are useful in cloning larger DNA fragments. The artificial chromosomes are of two types, namely, bacterial artificial chromosome and yeast artificial chromosome.
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Your goal is to produce black seeds resistant to mold. So you make the same cross again (between a homozygous black seeded, mold susceptible parent and a homozygous white seeded and mold resistant parent), and, again, advance progeny by SSD to create 100 F10 generation plants.
Based on the information you obtained from your first crossing experiment (Question #4), how many F10 plants would you expect to have black seeds and be resistant to mold?
Assume that a toxin produced by the mold fungus has been isolated. Only mold resistant seeds will germinate in the presence of the toxin. Could you use this toxin screening procedure to have segregation distortion work in your favor in the F2 generation? Explain your answer.
Info from Question 4
a. P Locus (Seed Color):
Hypothesis: The null hypothesis (H₀) is that seed color is controlled by alleles at a single locus.
Observed Data:
Total white seeds: 45 (resistant plants) + 6 (susceptible plants) = 51
Total black seeds: 7 (resistant…
10. Consider the following enzyme and its substrate where the "+" and "-" indicate cations and
anions, respectively. Explain which of the following inhibitors could inhibit this enzyme?
Which type of inhibitor would it be and why? (Video 5-2)
Substrate
Enzyme
Potential inhibitors
Using Punnett Squares Punnett squares are one good
way to predict the outcome of genetic crosses. Punnett
squares use mathematical probability to help predict the
genotype and phenotype combinations in genetic crosses. The
number of possible alleles from each parent determines the
number of rows and columns in the Punnett square.
Independent Assortment
KEY QUESTION How do alleles segregate when more than
one gene is involved?
Mendel wondered if the segregation of one pair of alleles
affects another pair. For example, does the gene that
determines the shape of a seed affect the gene for seed color?
This type of experiment is known as a two-factor, or dihybrid,
cross because it involves two different genes. Single-gene
crosses are monohybrid crosses.
Visual Reading Tool: Two-Factor Cross: F₂
The Punnett square shows the
results of self-crossing the F,
generation of a cross between
round yellow peas and wrinkled
green peas.
1. List the different genotypes in
the F, generation. What is the…
Chapter 11 Solutions
Brock Biology of Microorganisms (14th Edition)
Ch. 11.1 - Prob. 1MQCh. 11.1 - Prob. 2MQCh. 11.2 - Prob. 1MQCh. 11.2 - Prob. 2MQCh. 11.3 - Why is a primer needed at each end of the DNA...Ch. 11.3 - MINIQUIZ
• From which organisms are thermostable...Ch. 11.3 - How does RT-PCR differ from traditional PCR?Ch. 11.4 - What is the purpose of molecular cloning?Ch. 11.4 - Prob. 2MQCh. 11.4 - Prob. 3MQ
Ch. 11.5 - Prob. 1MQCh. 11.5 - How can site-directed mutagenesis be useful to...Ch. 11.5 - What are knockout mutations?Ch. 11.6 - Prob. 1MQCh. 11.6 - Prob. 2MQCh. 11.7 - Prob. 1MQCh. 11.7 - Prob. 2MQCh. 11.7 - Prob. 3MQCh. 11.8 - Prob. 1MQCh. 11.8 - Prob. 2MQCh. 11.9 - MINIQUIZ
• Describe the components needed for an...Ch. 11.9 - Prob. 2MQCh. 11.10 - Prob. 1MQCh. 11.10 - Prob. 2MQCh. 11.10 - Prob. 3MQCh. 11.11 - What major advantage does cloning mammalian genes...Ch. 11.11 - Prob. 2MQCh. 11.12 - Prob. 1MQCh. 11.12 - Prob. 2MQCh. 11.12 - Prob. 3MQCh. 11.13 - Prob. 1MQCh. 11.13 - Give an example of a genetically modified plant...Ch. 11.13 - How have transgenic salmon been engineered to...Ch. 11.14 - Explain why recombinant vaccines might be safer...Ch. 11.14 - Prob. 2MQCh. 11.15 - Explain why metagenomic cloning gives large...Ch. 11.15 - Prob. 2MQCh. 11.16 - Prob. 1MQCh. 11.16 - Prob. 2MQCh. 11.17 - What are biobricks?Ch. 11.17 - How was Escherichia coli modified to produce a...Ch. 11 - Prob. 1RQCh. 11 - Prob. 2RQCh. 11 - Describe the basic principles of gene...Ch. 11 - Prob. 4RQCh. 11 - Prob. 5RQCh. 11 - Prob. 6RQCh. 11 - Prob. 7RQCh. 11 - REVIEW QUESTIONS
8. What is a reporter gene?...Ch. 11 - Prob. 9RQCh. 11 - Prob. 10RQCh. 11 - Prob. 11RQCh. 11 - Prob. 12RQCh. 11 - Prob. 13RQCh. 11 - Prob. 14RQCh. 11 - Prob. 15RQCh. 11 - Prob. 16RQCh. 11 - Prob. 17RQCh. 11 - What is the Ti plasmid and how has it been of use...Ch. 11 - What is a subunit vaccine and why are subunit...Ch. 11 - How has metagenomics been used to find novel...Ch. 11 - Prob. 21RQCh. 11 - Prob. 22RQCh. 11 - Prob. 1AQCh. 11 - Suppose you have just determined the DNA base...Ch. 11 - Prob. 3AQCh. 11 - Prob. 4AQ
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- CHAPTER 12 LESSON 2 Applying Mendel's Principles READING TOOL Connect to Visuals Before you read, preview Figure 12-7. Try to infer the purpose of this diagram. As you read, compare your inference to the text. After you read, revise your statement if needed or write a new one about the diagram's purpose. Take notes on the lines provided. Then view the Punnett square and answer the questions below regarding the genotypes and phenotypes. Inference: Revision: Parent 2 rryy Gametes F ry Parent 1 RRYY Gametes RY RrYy The F, generation are all RrYy. 1. What is the phenotype of parent 1?. 2. What is the genotype of parent 1? 3. What is the phenotype of parent 2? 4. What is the genotype of parent 2? 5. What is the phenotype of the F, offspring?. 6. What is the genotype of the F, offspring?. 7. What kind of cross does this figure describe? 144 Chanter 12 Introduction to Genetice Copyright Pearson Education Inc. or its affiliator. All rights reserved.arrow_forwardHow is the term enzyme related to the term proteinarrow_forwardCan very low temperatures cause proteins to denature? Explain why or why not?arrow_forward
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