Brock Biology of Microorganisms (14th Edition)
14th Edition
ISBN: 9780321897398
Author: Michael T. Madigan, John M. Martinko, Kelly S. Bender, Daniel H. Buckley, David A. Stahl, Thomas Brock
Publisher: PEARSON
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Chapter 11, Problem 16RQ
Summary Introduction
The animal genome contains both exons (coding regions) and introns (non-coding regions). If the cloned gene possesses introns, it will affect the production of a correct protein molecule in a host. Hence, before cloning the DNA sequence must be modified (DNA sequence must contain only exons).
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Chapter 11 Solutions
Brock Biology of Microorganisms (14th Edition)
Ch. 11.1 - Prob. 1MQCh. 11.1 - Prob. 2MQCh. 11.2 - Prob. 1MQCh. 11.2 - Prob. 2MQCh. 11.3 - Why is a primer needed at each end of the DNA...Ch. 11.3 - MINIQUIZ
• From which organisms are thermostable...Ch. 11.3 - How does RT-PCR differ from traditional PCR?Ch. 11.4 - What is the purpose of molecular cloning?Ch. 11.4 - Prob. 2MQCh. 11.4 - Prob. 3MQ
Ch. 11.5 - Prob. 1MQCh. 11.5 - How can site-directed mutagenesis be useful to...Ch. 11.5 - What are knockout mutations?Ch. 11.6 - Prob. 1MQCh. 11.6 - Prob. 2MQCh. 11.7 - Prob. 1MQCh. 11.7 - Prob. 2MQCh. 11.7 - Prob. 3MQCh. 11.8 - Prob. 1MQCh. 11.8 - Prob. 2MQCh. 11.9 - MINIQUIZ
• Describe the components needed for an...Ch. 11.9 - Prob. 2MQCh. 11.10 - Prob. 1MQCh. 11.10 - Prob. 2MQCh. 11.10 - Prob. 3MQCh. 11.11 - What major advantage does cloning mammalian genes...Ch. 11.11 - Prob. 2MQCh. 11.12 - Prob. 1MQCh. 11.12 - Prob. 2MQCh. 11.12 - Prob. 3MQCh. 11.13 - Prob. 1MQCh. 11.13 - Give an example of a genetically modified plant...Ch. 11.13 - How have transgenic salmon been engineered to...Ch. 11.14 - Explain why recombinant vaccines might be safer...Ch. 11.14 - Prob. 2MQCh. 11.15 - Explain why metagenomic cloning gives large...Ch. 11.15 - Prob. 2MQCh. 11.16 - Prob. 1MQCh. 11.16 - Prob. 2MQCh. 11.17 - What are biobricks?Ch. 11.17 - How was Escherichia coli modified to produce a...Ch. 11 - Prob. 1RQCh. 11 - Prob. 2RQCh. 11 - Describe the basic principles of gene...Ch. 11 - Prob. 4RQCh. 11 - Prob. 5RQCh. 11 - Prob. 6RQCh. 11 - Prob. 7RQCh. 11 - REVIEW QUESTIONS
8. What is a reporter gene?...Ch. 11 - Prob. 9RQCh. 11 - Prob. 10RQCh. 11 - Prob. 11RQCh. 11 - Prob. 12RQCh. 11 - Prob. 13RQCh. 11 - Prob. 14RQCh. 11 - Prob. 15RQCh. 11 - Prob. 16RQCh. 11 - Prob. 17RQCh. 11 - What is the Ti plasmid and how has it been of use...Ch. 11 - What is a subunit vaccine and why are subunit...Ch. 11 - How has metagenomics been used to find novel...Ch. 11 - Prob. 21RQCh. 11 - Prob. 22RQCh. 11 - Prob. 1AQCh. 11 - Suppose you have just determined the DNA base...Ch. 11 - Prob. 3AQCh. 11 - Prob. 4AQ
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- During experimental RNAi, how does the researcher affect expression of a target gene? Group of answer choices Inject custom, lab-made double-stranded RNA into the specimen that will be targeted by the cell's existing Dicer to create siRNA complementary to the target gene's transcript. Inject lab-made Dicer proteins into the specimen which will cut up the target gene's transcript. Inject custom, lab-made siRNAs complementary to the target gene's transcript.arrow_forwardAlthough several different mammalian species have been cloned, the efficiency of this process is extremely low. Often tens or even hundreds of oocytes must be implanted with donor nuclei to obtain one healthy live birth. Many researchers believe the difficulties with cloning reside in the epigenetic modifications, such as DNA and histone methylation, that occur within various cells during an individual’s life. How do you suspect such modifications might affect the success of an experimentarrow_forwardCloning of a eukaryotic gene can be carried out in Escherichia coli. However, for the protein expression of an antioxidant gene from a donkey, a eukaryotic host cell is preferred. Why?arrow_forward
- How do we know that small noncoding RNA molecules canregulate gene expression?arrow_forwardWhy is the repression of genes by DNA methylation long-term?arrow_forwardDifferent forms of a protein are produced in the liver and in the brain by alternate splicing of its mRNA. How would a researcher clone the gene encoding that protein to study the form that was produced in the liver?arrow_forward
- What parts of the genome are reversibly methylated? How does this affect gene expression?arrow_forwardOne can synthesize RNA from any gene in a test tube (in vitro). You want to synthesize mRNA from a prokaryotic gene in a test tube. You added the gene, which contains its promoter, coding region, and rho-dependent transcription termination site, in a test tube. Which of the following do you need to add to this test tube so that you can synthesize RNA from this gene and terminate transcription accurately? 1) RNA polymerase core enzyme, 2) RNA polymerase holoenzyme,3) RNA polymerase II, 4) ATP, CTP, GTP, and TTP mixture 5) ATP, GTP, CTP, and UTP mixture, 6) dATP, dGTP, dCTP, and dUTP mixture 7) Rho factor, 8) primer. 3,4,7 1,6,7 2.5.7 2,4,6 2,5,7,8arrow_forwardHow does the presence of epigenetic modifications affect the accuracy and reliability of genetic analysis techniques such as DNA sequencing and PCR (Polymerase Chain Reaction)?arrow_forward
- Could quantitative PCR, which uses a DNA-binding dye, to show how many copies of the target DNA sequence could be used to quantify the amount of mRNA in a cell? Would you expect that a metabolically active tissue such as the liver would show more cDNA copies in such a method, compared to less metabolically active tissues such as skin cells? One reason that the types and amounts of mRNAs are quantified in different tissue types is to compare which genes are activated and which are inactive. It used to be thought that any gene that was transcribed was automatically translated. The discovery of RNA-degrading systems shows that the real situation in cells is more complemented. Do you believe that a larger amount of mRNA of a given type, say for alpha hemoglobin in immature red blood cells is a reliable indicator that more alpha hemoglobin protein will be made in those cells?arrow_forward2) what is negative control of genes ? Does it need active form or inactive form of the repressor ? 3) What is role of RNA polymerase, operator and the repressor in repressible operon and inducible operons ? 4) How is eukaryotic gene expression different than bacterial gene expression ? 5) How does histone acetylation influence transcription ?arrow_forwardWhich of the following methods is utilized by eukaryotes to control their gene expression that is different from the type of control found in bacteria? a) control of chromatic remodeling. b) control of RNA splicing. c) transcriptional control. d) control of both RNA splicing and chromatin remodeling.arrow_forward
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