Biology: The Unity and Diversity of Life (MindTap Course List)
14th Edition
ISBN: 9781305073951
Author: Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher: Cengage Learning
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Chapter 10, Problem 4CT
The photos above show flowers from Arabidopsis plants. One plant is wild-type (unmutated); the other carries a mutation in one of its ABC floral identity genes. This mutation causes sepals and petals to form instead of stamens and carpels. Refer to Figure 10.8 to decide which gene (A, B, or C) has been inactivated by the mutation.
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You conduct an experiment to study the expression of the S protein through the
tissues of your favorite plant (Arabidopsis thaliana). The morning of the experiment
you inject in the leaves a messenger RNA that codes for the synthesis of a single
protein made of two parts that are attached to each other: the functional S protein
and a red fluorescent protein tag (RFP). You perform two cross sections of the same
root, one section at the start of the experiment (time = Oh; corresponding to the time
of injection) and one section in the afternoon (time = 8h). Through fluorescent
microscopy you observe a change in the coloration inside the cells of the root's
central tissues (as indicated by the arrows): from no coloration (time = Oh) to red
(time = 8h).
• The cells of the central tissues in the roots do not have nuclei or ribosomes.
How can you explain this change of coloration? Please provide a cellular
feature that can lead to this.
• What is one advantage of using an RFP-tag in an mRNA?…
In roses, the synthesis of red pigment is produced by two steps in a pathway.
gene O
magenta intermediate -
gene P
colorless intermediate-
red pigment
What would the phenotype be of a plant homozygous for a null mutation of gene P?
What would the phenotype be of a plant homozygous for a null mutation of gene Q?
What would the phenotype be of a plant homozygous for null mutations of genes P and Q?
magenta
red
Match a genotype to each strain.
colorless
Strain
P locus Q locus
homozygous null mutation of gene P
homozygous null mutation of gene Q
homozygous null mutations of genes P and Q
Answer Bank
plp
PIP
What F2 ratio is expected from crossing a plant that is homozygous for a null mutation of gene P with a plant that is
homozygous for a null mutation of gene Q? Assume independent assortment.
9 colorless : 4 magenta : 3 red
9 red : 4 colorless : 3 magenta
O 9 red : 4 magenta : 3 colorless
You are a developmental geneticist studying flowering time variation in Arabidopsis. You perform a
mutagenesis screen to identify mutants in the photoperiod pathway. You conduct the screen and
find two different plants that show the same mutant phenotype. You then use a complementation
test. What is the predicted outcome of this test if both phenotypes are caused by mutations in
separate genes?
recover the wild type phenotype
overexpress the gene
O recover the mutant phenotype
Chapter 10 Solutions
Biology: The Unity and Diversity of Life (MindTap Course List)
Ch. 10 - The expression of a gene may depend on _______. a....Ch. 10 - Prob. 2SQCh. 10 - Binding of ______ to _______ in DNA can increase...Ch. 10 - Prob. 4SQCh. 10 - Prob. 5SQCh. 10 - Muscle cells differ from bone cells because...Ch. 10 - Prob. 7SQCh. 10 - Homeotic gene products _______. a. flank a...Ch. 10 - A gene that is knocked out is ________. a. deleted...Ch. 10 - Which of the following includes all of the others?...
Ch. 10 - Prob. 11SQCh. 10 - Effect of Paternal Grandmothers Food Supply on...Ch. 10 - Prob. 2DAACh. 10 - Effect of Paternal Grandmothers Food Supply on...Ch. 10 - Prob. 12SQCh. 10 - A cell with a Barr body is ___ . a. a bacterium b....Ch. 10 - Operons _____. a. only occur in bacteria b. have...Ch. 10 - Prob. 15SQCh. 10 - Why are some genes expressed and some not?Ch. 10 - Prob. 2CTCh. 10 - Almost all calico cats (one is pictured in FIGURE...Ch. 10 - The photos above show flowers from Arabidopsis...
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- Can you answer part a-c if its true or false a) the AP3 and PI show auto- and cross-regulatory interactions, as well as they form obligate heterodimers to carry out the B class gene function. Therefore, if there is no PI expression, AP3 expression alone is not sufficient for establishing the petal and stamen identities. b) Angiosperm is a group of plants whose seeds are borne within a mature ovary (fruit). c) The organ in different organisms under every variety of forms and functions due to evolutionary development from the same or a corresponding part in a common ancestor is homologous.arrow_forwardThe photos below show flowers from two Arabidopsis plants. One plant is wild-type unmutated; the other carries a mutation in one of its ABC floral identity genes. This mutation causes sepals and petals to form instead of stamens and carpels. Refer to Figure 10.7 to decide which gene A, B, or C has been inactivated by the mutation.arrow_forwardTrace the genetic steps in the development of a peanut plant that isresistant to insect larvae (table given). (Hint: You need to useAgrobacterium.)arrow_forward
- You are a developmental geneticist studying flowering time variation in Arabidopsis. You perform a mutagenesis screen to identify mutants in the photoperiod pathway. Given what you know about photoperiodism in Arabidopsis, what phenotype are you looking for and under what photoperiodic conditions would you perform the experiment? delayed flowering in long days delayed flowering in short days same flowering in short days early flowering in short days same flowering in long days early flowering in long daysarrow_forwardYou are studying a plant with tissue comprising both green and white sectors. You wish to decide whether this phenomenon is due (1) to a chloroplast mutation of the type considered in this chapter or (2) to a dominant nuclear mutation that inhibits chlorophyll production and is present only in certain tissue layers of the plant as a mosaic. Outline the experimental approach that you would use to resolve this problem.arrow_forwardIn roses, the synthesis of red pigment is by two steps ina pathway, as follows:colorless intermediate gene Pmagenta intermediate red pigment gene Qa. What would the phenotype be of a plant homozygousfor a null mutation of gene P?b. What would the phenotype be of a plant homozygousfor a null mutation of gene Q?c. What would the phenotype be of a plant homozygousfor null mutations of genes P and Q?d. Write the genotypes of the three strains in parts a, b,and c.e. What F2 ratio is expected from crossing plants fromparts a and b? (Assume independent assortment.)arrow_forward
- Yeast cells are grown with galactose as the sole carbon source and ATP levels are abundant. Describe and diagram how GAL1 gene expression will be changed (or unchanged) in 1) a ΔGal3 mutant and 2) a ΔGal4 mutant in comparison to WT. (Δ is a symbol for deletion.) WT: ΔGal3: ΔGal4:arrow_forwardWhich gene encodes the enzyme at the upper boxed step in the pathway? Which gene encodes the enzyme at the upper boxed step in the pathway? gene 1 gene 2 gene 3 gene 4arrow_forwardIn Figure 6-1,a. what do the yellow stars represent?b. explain in your own words why the heterozygote isfunctionally wild typearrow_forward
- please explain if it would be elongated or shortened expression! I know double mutant epistasis is always downstream> upstream but if upstream gene controls downstream, what happens?arrow_forwardIn Arabidopsis, it is well-known that a pulse of full-spectrum light during the night (in an otherwise long night) will induce flowering. This suggests that plants measure the length of night, and not the length of day. If the pulse of light during the night was blue light instead of full spectrum light, what would be the flowering time response of a plant with a knockout in cry2 (relative to wild type in the same conditions)? Explain.arrow_forwardYou identify an auxotroph that doesn't grow in normal medium, but does grow in medium supplemented with Q, indicating that it has a defect in one or more enzymes in this pathway. This auxotroph is also able to grow if you supplement the medium with D. Does this data support the one gene-one enzyme hypothesis? a yes, because only one enzyme is disrupted by the mutation b yes, because only one enzyme in the pathway is functional in the mutant strain c no, because multiple enzymes in the pathway could be functional in the mutant strain d no, because multiple enzymes could be disrupted by the mutationarrow_forward
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