Chapter 10, Problem 22P

Summary Introduction

To analyze:

Observe following DNA sequence:

$\begin{array}{l}\text{5'-TTCGAATTCGACTCAGGATCCTACAAGTTTCAT-3'}\hfill \\ \text{3'-AAGCTTAAGCTGAGTCCTAGGATGTTCAAACTA-5'}\hfill \end{array}$

Identify the restriction sites from the given sequence and highlight it using a box around each site.

a. EcoRI ($\text{5'-GAATTC-3'}$)

b. BamHI ($\text{5'-GGATCC-3'}$)

c. HinIII ($\text{5'-AAGCTT-3'}$)

Introduction:

The enzymes which are able to spot a specific sequence on the DNA and are able to cut these specific sites are called as Restriction endonucleases. The sites where these restriction endonucleases introduce a cut on the DNA molecule are called as Restriction sites. The specificity of the restriction enzymes has led to the identification of several restriction enzymes which has its extensive use in research and industrial applications.