Question asked to describe the molecular parameter that causes DNA fragments to have different electrophoretic mobility. Question asked to describe the parameter that causes different mobilities in mRNA. Question asked to describe the parameters that cause different mobilities in proteins. Introduction: Electrophoresis is an experimental approach for the separation of protein, DNA, and mRNA. The separation can be done on the basis of molecular weight, shape, and charge of protein DNA and mRNA under the electric field. Separation of proteins can be represented by the mathematical equation: μ e = q 6μηr Here, μ e = Mobility of sample in electrophoreses. q = Charge of sample. η = Given solution viscosity. r = Radius of sample. There are two different types of electrophoresis i.e. Polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate (SDS). The moment of a molecule depends on the pore size of the gel.

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Answer 1

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Definition Definition Phase during which the number of bacteria cell deaths gradually equals the number of viable or actively dividing cells due to decreased reproduction rate. Microbes utilize all nutrients present in the growth medium to multiply during the growth of the bacterial population, which results in the deposition of waste matter, toxins, and inhibitory compounds in the media. This changes media conditions, such as temperature and pH, creating an unfavorable environment for bacterial growth. Here, the growth rate becomes stabilized.

Chapter 10, Problem 10P

Summary Introduction

To analyze:

Question asked to describe the molecular parameter that causes DNA fragments to have different electrophoretic mobility.

Question asked to describe the parameter that causes different mobilities in mRNA.

Question asked to describe the parameters that cause different mobilities in proteins.

Introduction:

Electrophoresis is an experimental approach for the separation of protein, DNA, and mRNA. The separation can be done on the basis of molecular weight, shape, and charge of protein DNA and mRNA under the electric field. Separation of proteins can be represented by the mathematical equation:

μe = q6μηr

Here,

μe = Mobility of sample in electrophoreses.

q = Charge of sample.

η = Given solution viscosity.

r = Radius of sample.

There are two different types of electrophoresis i.e. Polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate (SDS). The moment of a molecule depends on the pore size of the gel.

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For short answer questions, write your answers on the line provided. To the right is the mRNA codon table to use as needed throughout the exam. First letter U บบบ U CA UUCPhe UUA UCU Phe UCC UUG Leu CUU UAU. G U UAC TV UGCys UAA Stop UGA Stop A UAG Stop UGG Trp Ser UCA UCG CCU] 0 CUC CUA CCC CAC CAU His CGU CGC Leu Pro CCA CAA Gin CGA Arg CUG CCG CAG CGG AUU ACU AAU T AUC lle A 1 ACC Thr AUA ACA AUG Mot ACG AGG Arg GUU GCU GUC GCC G Val Ala GAC Asp GGU GGC GUA GUG GCA GCG GAA GGA Gly Glu GAGJ GGG AACASH AGU Ser AAA1 AAG Lys GAU AGA CAL CALUCAO CAO G Third letter 1. (+7) Use the table below to answer the questions; use the codon table above to assist you. The promoter sequence of DNA is on the LEFT. You do not need to fill in the entire table. Assume we are in the middle of a gene sequence (no need to find a start codon). DNA 1 DNA 2 mRNA tRNA Polypeptide C Val G C. T A C a. On which strand of DNA is the template strand (DNA 1 or 2)?_ b. On which side of the mRNA is the 5' end (left or…

3. (6 pts) Fill in the boxes according to the directions on the right. Structure R-C R-COOH OH R-OH i R-CO-R' R R-PO4 R-CH3 C. 0 R' R-O-P-OH 1 OH H R-C-H R-N' I- H H R-NH₂ \H Name Properties

4. (6 pts) Use the molecule below to answer these questions and identify the side chains and ends. Please use tidy boxes to indicate parts and write the letter labels within that box. a. How many monomer subunits are shown? b. Box a Polar but non-ionizable side chain and label P c. Box a Basic Polar side chain and label BP d. Box the carboxyl group at the end of the polypeptide and label with letter C (C-terminus) H H OHHO H H 0 HHO H-N-CC-N-C-C N-C-C-N-GC-OH I H-C-H CH2 CH2 CH2 H3C-C+H CH2 CH2 OH CH CH₂ C=O OH CH2 NH2

Answer 2

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Definition Definition Phase during which the number of bacteria cell deaths gradually equals the number of viable or actively dividing cells due to decreased reproduction rate. Microbes utilize all nutrients present in the growth medium to multiply during the growth of the bacterial population, which results in the deposition of waste matter, toxins, and inhibitory compounds in the media. This changes media conditions, such as temperature and pH, creating an unfavorable environment for bacterial growth. Here, the growth rate becomes stabilized.

Chapter 10, Problem 10P

Summary Introduction

To analyze:

Question asked to describe the molecular parameter that causes DNA fragments to have different electrophoretic mobility.

Question asked to describe the parameter that causes different mobilities in mRNA.

Question asked to describe the parameters that cause different mobilities in proteins.

Introduction:

Electrophoresis is an experimental approach for the separation of protein, DNA, and mRNA. The separation can be done on the basis of molecular weight, shape, and charge of protein DNA and mRNA under the electric field. Separation of proteins can be represented by the mathematical equation:

μe = q6μηr

Here,

μe = Mobility of sample in electrophoreses.

q = Charge of sample.

η = Given solution viscosity.

r = Radius of sample.

There are two different types of electrophoresis i.e. Polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate (SDS). The moment of a molecule depends on the pore size of the gel.

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Check out a sample textbook solution

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Answer 3

Question

Definition Definition Phase during which the number of bacteria cell deaths gradually equals the number of viable or actively dividing cells due to decreased reproduction rate. Microbes utilize all nutrients present in the growth medium to multiply during the growth of the bacterial population, which results in the deposition of waste matter, toxins, and inhibitory compounds in the media. This changes media conditions, such as temperature and pH, creating an unfavorable environment for bacterial growth. Here, the growth rate becomes stabilized.

Chapter 10, Problem 10P

Summary Introduction

To analyze:

Question asked to describe the molecular parameter that causes DNA fragments to have different electrophoretic mobility.

Question asked to describe the parameter that causes different mobilities in mRNA.

Question asked to describe the parameters that cause different mobilities in proteins.

Introduction:

Electrophoresis is an experimental approach for the separation of protein, DNA, and mRNA. The separation can be done on the basis of molecular weight, shape, and charge of protein DNA and mRNA under the electric field. Separation of proteins can be represented by the mathematical equation:

μe = q6μηr

Here,

μe = Mobility of sample in electrophoreses.

q = Charge of sample.

η = Given solution viscosity.

r = Radius of sample.

There are two different types of electrophoresis i.e. Polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate (SDS). The moment of a molecule depends on the pore size of the gel.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 4

Question

Definition Definition Phase during which the number of bacteria cell deaths gradually equals the number of viable or actively dividing cells due to decreased reproduction rate. Microbes utilize all nutrients present in the growth medium to multiply during the growth of the bacterial population, which results in the deposition of waste matter, toxins, and inhibitory compounds in the media. This changes media conditions, such as temperature and pH, creating an unfavorable environment for bacterial growth. Here, the growth rate becomes stabilized.

Chapter 10, Problem 10P

Summary Introduction