Chapter 10, Problem 12P

Summary Introduction

To analyze:

Why the positions of nucleic acid bands observed in one kind of blot cannot be directly compared with those in the other.

Introduction:

Blotting is the technique routinely used for the detection of biomolecules such as DNA, RNA, and proteins, named as Southern blotting, northern blotting, and western blotting respectively. In southern and northern blot method, the DNA and RNA are separated by agarose gel electrophoresis and then detected by using the DNA probe complementary to the targeted sample. In autoradiography, the radioactively labelled substances with radioisotopes are used. In a bio-analytical procedure, such substances that are distributed in a biological sample are visualized.