Chapter 10, Problem 13P

shows three different sequences of the same autosome in human populations. These sequences are each from a single chromatid. You know this to be true because the PCR amplifications were from individual haploid gametes. If you wanted to obtain the same information by PCR amplification of genomic DNA from somatic cells, the problem would be somewhat more complicated because the starting cells are diploid. Each PCR product to be sequenced would thus actually be amplified from two homologous chromosomes. You could still verify the existence of the three different haploid sequences shown in Problem 8 by analyzing the somatic genomic DNA from as few as two people (if they happened to be the right people).

a.	Indicate the diploid genotypes of two people from whom you could identify these three different haploid sequences. Account for all 10 nucleotides in the sequences. Three possible correct answers exist; you need to show one.
b.	If you PCR amplified DNA from somatic genomic DNA from a person with one particular genotype, you would not be able to conclude that their genome contains any of these three sequences. What is the genotype of this person? Explain why you could not reach this conclusion.