a. To determine: The reason that 18- nucleotide -long primers used in question 11 can amplify the exon of CFTR gene. Introduction: Polymerase chain reaction (PCR) may be defined as a molecular technique used for the amplification of DNA upto many folds. The three main steps of PCR reactions are denaturation, annealing, and extension.

Question

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Answer 1

Question

Chapter 10, Problem 11P

Summary Introduction

a.

To determine:

The reason that 18-nucleotide-long primers used in question 11 can amplify the exon of CFTR gene.

Introduction:

Polymerase chain reaction (PCR) may be defined as a molecular technique used for the amplification of DNA upto many folds. The three main steps of PCR reactions are denaturation, annealing, and extension.

Summary Introduction

b.

To determine:

The reason that lower limit of primer used in PCR is 16 nucleotides only.

Introduction:

Primers are the short oligonucleotides of DNA, and the approximate length of primers are 16-30 nucleotides. These primers can be synthesized biochemically or chemically.

Summary Introduction

c.

To determine:

Whether the PCR product is mismatched at the 5' end or 3' end of the primer if one of the primers has single base pair mismatch.

Introduction:

The length of the human genome is 3.0×10⁹ base pairs. There is only one chance that a complementary sequence used in PCR can be used as a primer.

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Answer 2

Question

Chapter 10, Problem 11P

Summary Introduction

a.

To determine:

The reason that 18-nucleotide-long primers used in question 11 can amplify the exon of CFTR gene.

Introduction:

Polymerase chain reaction (PCR) may be defined as a molecular technique used for the amplification of DNA upto many folds. The three main steps of PCR reactions are denaturation, annealing, and extension.

Summary Introduction

b.

To determine:

The reason that lower limit of primer used in PCR is 16 nucleotides only.

Introduction:

Primers are the short oligonucleotides of DNA, and the approximate length of primers are 16-30 nucleotides. These primers can be synthesized biochemically or chemically.

Summary Introduction

c.

To determine:

Whether the PCR product is mismatched at the 5' end or 3' end of the primer if one of the primers has single base pair mismatch.

Introduction:

The length of the human genome is 3.0×10⁹ base pairs. There is only one chance that a complementary sequence used in PCR can be used as a primer.

Expert Solution & Answer

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Answer 3

Question

Chapter 10, Problem 11P

Summary Introduction

a.

To determine:

The reason that 18-nucleotide-long primers used in question 11 can amplify the exon of CFTR gene.

Introduction:

Polymerase chain reaction (PCR) may be defined as a molecular technique used for the amplification of DNA upto many folds. The three main steps of PCR reactions are denaturation, annealing, and extension.

Summary Introduction

b.

To determine:

The reason that lower limit of primer used in PCR is 16 nucleotides only.

Introduction:

Primers are the short oligonucleotides of DNA, and the approximate length of primers are 16-30 nucleotides. These primers can be synthesized biochemically or chemically.

Summary Introduction

c.

To determine:

Whether the PCR product is mismatched at the 5' end or 3' end of the primer if one of the primers has single base pair mismatch.

Introduction:

The length of the human genome is 3.0×10⁹ base pairs. There is only one chance that a complementary sequence used in PCR can be used as a primer.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 10, Problem 11P

Summary Introduction

a.

To determine:

The reason that 18-nucleotide-long primers used in question 11 can amplify the exon of CFTR gene.

Introduction:

Polymerase chain reaction (PCR) may be defined as a molecular technique used for the amplification of DNA upto many folds. The three main steps of PCR reactions are denaturation, annealing, and extension.

Summary Introduction

b.

To determine:

The reason that lower limit of primer used in PCR is 16 nucleotides only.

Introduction:

Primers are the short oligonucleotides of DNA, and the approximate length of primers are 16-30 nucleotides. These primers can be synthesized biochemically or chemically.

Summary Introduction

c.

To determine:

Whether the PCR product is mismatched at the 5' end or 3' end of the primer if one of the primers has single base pair mismatch.

Introduction:

The length of the human genome is 3.0×10⁹ base pairs. There is only one chance that a complementary sequence used in PCR can be used as a primer.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 5

Chapter 10, Problem 11P

Summary Introduction

a.

To determine:

The reason that 18-nucleotide-long primers used in question 11 can amplify the exon of CFTR gene.

Introduction:

Polymerase chain reaction (PCR) may be defined as a molecular technique used for the amplification of DNA upto many folds. The three main steps of PCR reactions are denaturation, annealing, and extension.

Summary Introduction

b.

To determine:

The reason that lower limit of primer used in PCR is 16 nucleotides only.

Introduction:

Primers are the short oligonucleotides of DNA, and the approximate length of primers are 16-30 nucleotides. These primers can be synthesized biochemically or chemically.

Summary Introduction

c.

To determine:

Whether the PCR product is mismatched at the 5' end or 3' end of the primer if one of the primers has single base pair mismatch.

Introduction:

The length of the human genome is 3.0×10⁹ base pairs. There is only one chance that a complementary sequence used in PCR can be used as a primer.

Answer 6

Chapter 10, Problem 11P

Summary Introduction

a.

To determine:

The reason that 18-nucleotide-long primers used in question 11 can amplify the exon of CFTR gene.

Introduction:

Polymerase chain reaction (PCR) may be defined as a molecular technique used for the amplification of DNA upto many folds. The three main steps of PCR reactions are denaturation, annealing, and extension.

Answer 7

Chapter 10, Problem 11P

Summary Introduction

a.

To determine:

The reason that 18-nucleotide-long primers used in question 11 can amplify the exon of CFTR gene.

Introduction:

Polymerase chain reaction (PCR) may be defined as a molecular technique used for the amplification of DNA upto many folds. The three main steps of PCR reactions are denaturation, annealing, and extension.

Answer 8

Summary Introduction

b.

To determine:

The reason that lower limit of primer used in PCR is 16 nucleotides only.

Introduction:

Primers are the short oligonucleotides of DNA, and the approximate length of primers are 16-30 nucleotides. These primers can be synthesized biochemically or chemically.

Answer 9

Summary Introduction

b.

To determine:

The reason that lower limit of primer used in PCR is 16 nucleotides only.

Introduction:

Primers are the short oligonucleotides of DNA, and the approximate length of primers are 16-30 nucleotides. These primers can be synthesized biochemically or chemically.

Answer 10

Summary Introduction

c.

To determine:

Whether the PCR product is mismatched at the 5' end or 3' end of the primer if one of the primers has single base pair mismatch.

Introduction:

The length of the human genome is 3.0×10⁹ base pairs. There is only one chance that a complementary sequence used in PCR can be used as a primer.

Answer 11

Summary Introduction

c.

To determine:

Whether the PCR product is mismatched at the 5' end or 3' end of the primer if one of the primers has single base pair mismatch.

Introduction:

The length of the human genome is 3.0×10⁹ base pairs. There is only one chance that a complementary sequence used in PCR can be used as a primer.

Answer 12

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Answer 13

Expert Solution & Answer

Answer 14

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Answer 15

Ch. 10 - Choose the phrase from the right column that best...Ch. 10 - Would you characterize the pattern of inheritance...Ch. 10 - Would you be more likely to find single nucleotide...Ch. 10 - A recent estimate of the rate of base...Ch. 10 - If you examine Fig. 10.5 on p. 344 closely, you...Ch. 10 - Approximately 50 million SNPs have thus far been...Ch. 10 - Mutations at simple sequence repeat SSR loci occur...Ch. 10 - Humans and gorillas last shared a common ancestor...Ch. 10 - Using PCR, you want to amplify an approximately 1...Ch. 10 - Prob. 10P

Solution Summary: The author explains that the 18-nucleotide-long primers used in question 11 can amplify the exon of CFTR gene.

Concept explainers

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b.

To determine:

The reason that lower limit of primer used in PCR is 16 nucleotides only.

Introduction:

Primers are the short oligonucleotides of DNA, and the approximate length of primers are 16-30 nucleotides. These primers can be synthesized biochemically or chemically.

Want to see the full answer?

Chapter 10 Solutions

Solution Summary: The author explains that the 18-nucleotide-long primers used in question 11 can amplify the exon of CFTR gene.

Concept explainers

Videos

b. To determine: The reason that lower limit of primer used in PCR is 16 nucleotides only. Introduction: Primers are the short oligonucleotides of DNA, and the approximate length of primers are 16-30 nucleotides. These primers can be synthesized biochemically or chemically.

Want to see the full answer?

Chapter 10 Solutions

b.

To determine:

The reason that lower limit of primer used in PCR is 16 nucleotides only.

Introduction:

Primers are the short oligonucleotides of DNA, and the approximate length of primers are 16-30 nucleotides. These primers can be synthesized biochemically or chemically.