Without Inhibitor [substrate], mM 1 Rate (V.), mM/min 0.997774599 1.076855826 With Inhibitor Rate (Vo), mM/min 0.847401 1.5 0.973852 2 1.122873947 1.037991 2.5 1.160626292 1.068431 3 1.184072485 1.105866

Without Inhibitor [substrate], mM 1 Rate (V.), mM/min 0.997774599 1.076855826 With Inhibitor Rate (Vo), mM/min 0.847401 1.5 0.973852 2 1.122873947 1.037991 2.5 1.160626292 1.068431 3 1.184072485 1.105866

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter6: Oral Medication Labels And Dosage Calculation

Section: Chapter Questions

Problem 2.7P

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Question

Another member of your research group studied the kinetics of the
GAPDH from the organism. They also determined if the GAPDH from
the organism is also inhibited by the known inhibitor of GAPDH from
humans.

From the following data, determine the KM (Michaelis-Menten Constant) and the Vmax
(maximum velocity) of the enzyme without and with the inhibitor.

Without Inhibitor
[substrate], mM
1
Rate (V.), mM/min
0.997774599
1.076855826
With Inhibitor
Rate (Vo), mM/min
0.847401
1.5
0.973852
2
1.122873947
1.037991
2.5
1.160626292
1.068431
3
1.184072485
1.105866

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