You obtained the following raw data when setting up a Biuret standard curve:AbsorbancyBSA (mg/ml)540nm00.15810.21020.26030.30540.36050.41060.45570.51080.5309100.5500.554After blanking against a biuret-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancyof 0.284 was obtained.Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3significant figures).

Proteins are large biomolecules made up of amino acid residues linked via a peptide bond. Amino…

Answered: You obtained the following raw data…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

Calculate the appropriate volume (in µL) of 9X loading buffer that should be added to 46.0 µL of a sample prior to loading the sample on an agarose gel.
A calibration curve for the absorbance of an unknown is shown below. What would be the concentration of a sample with absorbance of 0.45. Give the answer with the correct unit.
A series of bovine serum albumin (BSA) was prepared and 1 mL of each solution was subjected to a Bradford assay. The increase in absorbance at 595 nm relative to the concentration of protein was plotted as shown below. Save 1.0- 0.8- 0.6- 0.4- 0.2- 0.04 0.0 0.5 1.0 2.0 2.5 1.5 BSA concentration (mg/mL) a) Using the graph, calculate the original concentration of protein present in a mixture of haemoglobin and methylene blue (diluted 1:50), which gave an absorbance of 0.20 at a wavelength of 595 nm using the Bradford assay. Show all workings. Absorbance (O.D.)
A dosage of 3 mcg/kg/min has been ordered for an 87.4 kg adult. The solution has a strength of 50 mg in 250 mL. Calculate the dosage per min.
Construct a standard curve (absorbance versus protein concentration in the standards) Conc. (mg/ml) 0 0.25 0.5 0.75 1.0 Absorbance 0.000 0.126 0.249 0.361 0.464
Laemmli buffer, also referred to as SDS-reducing buffer, is added to each of the samples. It is a blue solution and the recipe for 2X Laemmli buffer is: 0.125 M Tris HCl, pH 6.8, 20% glycerol, 4% SDS and 10% β-mercaptoethanol (βME). What is the purpose of each of these ingredients?
It takes 175ml of 0.75 M H2SO4 to completely neutralize 285 ml of NaOH. What is the concentration of the NaOH solution?
A pharmacist is preparing a compounded oral suspension preparation using commercially available tablets as the source of the active pharmaceutical ingredient (API). The recipe is as follows: "API" 0.8 mg/mL Structured suspension vehicle qs ad 180 mL Sig: 2 tsp PO BID PRN The tablets are available at a strength of 25 mg. Assume that when the the minimum number of tablets is used to prepare this compounded suspension, the crushed tablets take up 9.5 mL of volume.What volume of suspension structured vehicle will be needed to prepare this compounded oral solution?Round the answer to one decimal place.
A pharmacist is preparing a compounded oral suspension preparation using commercially available tablets as the source of the active pharmaceutical ingredient (API). The recipe is as follows: "API" 0.8 mg/mL Structured suspension vehicle qs ad 240 mL Sig: 1 tsp PO BID PRN The tablets are available at a strength of 20 mg. Assume that when the the minimum number of tablets is used to prepare this compounded suspension, the crushed tablets take up 12.2 mL of volume.What volume of suspension structured vehicle will be needed to prepare this compounded oral solution?Round the answer to one decimal place.
KCI for injection contains 40 mEq in 20 mL. An order requires 15 mEq, how many mL are needed?
BSA Volume (1.0mg/ml) (ul) [BSA] (ug/ml) Absorbance 595nm Net Abs 595nm 0.0 0.371 10.0 0.559 20.0 .707 25.0 .902 Please help me calculate BSA and Net Abs (explaination and details pls)
Using a P-1000 micropipette, you transfer 800 microliter of ddH2O to a beaker and measure its weight. It turns out to be only 780 milligram; what is the percent error of this micropipette?

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