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3. In your textbook the termi- nal enzyme catalyzing the ter- minal step of glycolysis is known as pyruvate kinase (PK). No indication is given that in mammalian tissues there are four different iso- forms of this enzyme. The four mammalian isoforms are known as PKL, PKR, PKM1 and PKM2. PKL is expressed in the liver, and PKR is ex- pressed in red blood cells. C PKM1 and PKM2 are derived from altemative splicing of the PKM gene. PKM1 is a constitu- tively active isoform expressed Active Site A PKM2 Monomer B Fructose-1,6-Bisphosphate N Phe, Ala, Ser Binding Site PKM2 Tetramer "Activator" Binding Site in differentiated cells from various tissues. In contrast, PKM2 has low basal activity and is activated by an effector molecule fructose 1,6-bisphosphate (FBP). PKM2 is expressed in most proliferating cells. In addition, the regulation of PKM2 pyruvate kinase activity plays an essential role in cancer metabolism and is crucial for the growth and survival of cancer cells. Recently, PKM2 has been reported to function also as a nuclear protein kinase to regulate gene transcription and promote tumorigenesis. The diagram on the right illustrates the quaternary structure of the PKM2 tetramer and the binding sites of metabolic effector molecules. (a) In the laboratory, the catalytic activity of pyruvate kinase is measured by a continuous assay coupled to lactate dehy-drogenase because the reaction products of the PK reac- tion have no suitable absorption or fluorescence properties, in contrast to the properties of NADH and NAD*, as shown in the diagram on the right. Write both reactions with structural for- mulas as a coupled system naming all metabolites, cofactors, and enzymes. Under what conditions in the cell do these reac- tions occur and why is this important for continued turnover of glycolysis? Optical density NAD+ NADH 250 300 350 wavelength (nm) 400

3. In your textbook the termi- nal enzyme catalyzing the ter- minal step of glycolysis is known as pyruvate kinase (PK). No indication is given that in mammalian tissues there are four different iso- forms of this enzyme. The four mammalian isoforms are known as PKL, PKR, PKM1 and PKM2. PKL is expressed in the liver, and PKR is ex- pressed in red blood cells. C PKM1 and PKM2 are derived from altemative splicing of the PKM gene. PKM1 is a constitu- tively active isoform expressed Active Site A PKM2 Monomer B Fructose-1,6-Bisphosphate N Phe, Ala, Ser Binding Site PKM2 Tetramer "Activator" Binding Site in differentiated cells from various tissues. In contrast, PKM2 has low basal activity and is activated by an effector molecule fructose 1,6-bisphosphate (FBP). PKM2 is expressed in most proliferating cells. In addition, the regulation of PKM2 pyruvate kinase activity plays an essential role in cancer metabolism and is crucial for the growth and survival of cancer cells. Recently, PKM2 has been reported to function also as a nuclear protein kinase to regulate gene transcription and promote tumorigenesis. The diagram on the right illustrates the quaternary structure of the PKM2 tetramer and the binding sites of metabolic effector molecules. (a) In the laboratory, the catalytic activity of pyruvate kinase is measured by a continuous assay coupled to lactate dehy-drogenase because the reaction products of the PK reac- tion have no suitable absorption or fluorescence properties, in contrast to the properties of NADH and NAD*, as shown in the diagram on the right. Write both reactions with structural for- mulas as a coupled system naming all metabolites, cofactors, and enzymes. Under what conditions in the cell do these reac- tions occur and why is this important for continued turnover of glycolysis? Optical density NAD+ NADH 250 300 350 wavelength (nm) 400

Biochemistry
Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter27: Metabolic Integration And Organ Specialization
Section: Chapter Questions
Problem 5P
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3. In your textbook the termi- nal enzyme catalyzing the ter- minal step of glycolysis is known as pyruvate kinase (PK). No indication is given that in mammalian tissues there are four different iso- forms of this enzyme. The four mammalian isoforms are known as PKL, PKR, PKM1 and PKM2. PKL is expressed in the liver, and PKR is ex- pressed in red blood cells. C PKM1 and PKM2 are derived from altemative splicing of the PKM gene. PKM1 is a constitu- tively active isoform expressed Active Site A PKM2 Monomer B Fructose-1,6-Bisphosphate N Phe, Ala, Ser Binding Site PKM2 Tetramer "Activator" Binding Site in differentiated cells from various tissues. In contrast, PKM2 has low basal activity and is activated by an effector molecule fructose 1,6-bisphosphate (FBP). PKM2 is expressed in most proliferating cells. In addition, the regulation of PKM2 pyruvate kinase activity plays an essential role in cancer metabolism and is crucial for the growth and survival of cancer cells. Recently, PKM2 has been reported to function also as a nuclear protein kinase to regulate gene transcription and promote tumorigenesis. The diagram on the right illustrates the quaternary structure of the PKM2 tetramer and the binding sites of metabolic effector molecules. (a) In the laboratory, the catalytic activity of pyruvate kinase is measured by a continuous assay coupled to lactate dehy-drogenase because the reaction products of the PK reac- tion have no suitable absorption or fluorescence properties, in contrast to the properties of NADH and NAD*, as shown in the diagram on the right. Write both reactions with structural for- mulas as a coupled system naming all metabolites, cofactors, and enzymes. Under what conditions in the cell do these reac- tions occur and why is this important for continued turnover of glycolysis? Optical density NAD+ NADH 250 300 350 wavelength (nm) 400
Transcribed Image Text:3. In your textbook the termi- nal enzyme catalyzing the ter- minal step of glycolysis is known as pyruvate kinase (PK). No indication is given that in mammalian tissues there are four different iso- forms of this enzyme. The four mammalian isoforms are known as PKL, PKR, PKM1 and PKM2. PKL is expressed in the liver, and PKR is ex- pressed in red blood cells. C PKM1 and PKM2 are derived from altemative splicing of the PKM gene. PKM1 is a constitu- tively active isoform expressed Active Site A PKM2 Monomer B Fructose-1,6-Bisphosphate N Phe, Ala, Ser Binding Site PKM2 Tetramer "Activator" Binding Site in differentiated cells from various tissues. In contrast, PKM2 has low basal activity and is activated by an effector molecule fructose 1,6-bisphosphate (FBP). PKM2 is expressed in most proliferating cells. In addition, the regulation of PKM2 pyruvate kinase activity plays an essential role in cancer metabolism and is crucial for the growth and survival of cancer cells. Recently, PKM2 has been reported to function also as a nuclear protein kinase to regulate gene transcription and promote tumorigenesis. The diagram on the right illustrates the quaternary structure of the PKM2 tetramer and the binding sites of metabolic effector molecules. (a) In the laboratory, the catalytic activity of pyruvate kinase is measured by a continuous assay coupled to lactate dehy-drogenase because the reaction products of the PK reac- tion have no suitable absorption or fluorescence properties, in contrast to the properties of NADH and NAD*, as shown in the diagram on the right. Write both reactions with structural for- mulas as a coupled system naming all metabolites, cofactors, and enzymes. Under what conditions in the cell do these reac- tions occur and why is this important for continued turnover of glycolysis? Optical density NAD+ NADH 250 300 350 wavelength (nm) 400
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