What is the method of MS protein quantification presented here

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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What is the method of MS protein quantification presented here?

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Cell state 1
Cell state 2
Cell culture with
Cell culture plus,
for example,
'heavy' arginine
normal arginine
Combine cell lysates
and purify proteins
Protein digestion
LC-tandem-MS
of labelled peptides
"Light' peptide 'Heavy' peptide
Quantify levels
from peak ratio
m/z
Intensity
Transcribed Image Text:Cell state 1 Cell state 2 Cell culture with Cell culture plus, for example, 'heavy' arginine normal arginine Combine cell lysates and purify proteins Protein digestion LC-tandem-MS of labelled peptides "Light' peptide 'Heavy' peptide Quantify levels from peak ratio m/z Intensity
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