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You are trying to purify Protein 1 from a mix of 4 different proteins without denaturing the protein. Protein 1 has a molecular weight of 3,000 Daltons and is very rich in lysine (Lys: K) Protein 2 has a molecular weight of 30,000 Daltons and is very rich in aspartic acid (Asp: D) Protein 3 has a molecular weight of 300,000 Daltons and is very rich in arginine (Arg: R) Protein 4 has a molecular weight of 30,000 Daltons and is very rich in histidine (His: H) What type of matrix/technique would make the most sense to use? NH, CH, NH NH, C-NH, NH CH2 CH, H,N*-C-C NH CH2 CH2 H,N-C CH, CH, CH2 H,N-C- H,N-C H. H. H Aspartic acid (Asp or D) Histidine Lysine (Lys or K) Arginine (Arg or R) (His or H) Oon exchange chromatography Oan affevity matrix that binds to protein 3 only Oion exchange chromatography Oan affinity matrix that binds to protein 3 only OSDS-poly acrylamide gel electrophoresis Oan affinity matrix that binds to all 4 proteins equally Ogel filtration

You are trying to purify Protein 1 from a mix of 4 different proteins without denaturing the protein. Protein 1 has a molecular weight of 3,000 Daltons and is very rich in lysine (Lys: K) Protein 2 has a molecular weight of 30,000 Daltons and is very rich in aspartic acid (Asp: D) Protein 3 has a molecular weight of 300,000 Daltons and is very rich in arginine (Arg: R) Protein 4 has a molecular weight of 30,000 Daltons and is very rich in histidine (His: H) What type of matrix/technique would make the most sense to use? NH, CH, NH NH, C-NH, NH CH2 CH, H,N*-C-C NH CH2 CH2 H,N-C CH, CH, CH2 H,N-C- H,N-C H. H. H Aspartic acid (Asp or D) Histidine Lysine (Lys or K) Arginine (Arg or R) (His or H) Oon exchange chromatography Oan affevity matrix that binds to protein 3 only Oion exchange chromatography Oan affinity matrix that binds to protein 3 only OSDS-poly acrylamide gel electrophoresis Oan affinity matrix that binds to all 4 proteins equally Ogel filtration

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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You are trying to purify Protein 1 from a mix of 4 different proteins without denaturing the protein. Protein 1 has a molecular weight of 3,000 Daltons and is very rich in lysine (Lys; K) Protein 2 has a molecular weight of 30,000 Daltons and is very rich in aspartic acid (Asp: D) Protein 3 has a molecular weight of 300,000 Daltons and is very rich in arginine (Arg: R) Protein 4 has a molecular weight of 30,000 Daltons and is very rich in histidine (His: H) What type of matrix/technique would make the most sense to use? NH, CH2 CH2 CH2 NH NH2 C-NH, NH NH CH, H,N-C CH, CH2 CH2 H,N-C H,N*-C- H. H. H,N-C C H H Aspartic acid (Asp or D) Arginine (Arg or R) Histidine Lysine (Lys or K) (His or H) Olon exchange chromatography Oan affnity matrix that binds to protein 3 only Oion exchange chromatography Oan affinity matrix that binds to protein 3 only OSDS-poly acrylamide gel electrophoresis Oan affinity matrix that binds to all 4 proteins equally Ogel filtration
Transcribed Image Text:You are trying to purify Protein 1 from a mix of 4 different proteins without denaturing the protein. Protein 1 has a molecular weight of 3,000 Daltons and is very rich in lysine (Lys; K) Protein 2 has a molecular weight of 30,000 Daltons and is very rich in aspartic acid (Asp: D) Protein 3 has a molecular weight of 300,000 Daltons and is very rich in arginine (Arg: R) Protein 4 has a molecular weight of 30,000 Daltons and is very rich in histidine (His: H) What type of matrix/technique would make the most sense to use? NH, CH2 CH2 CH2 NH NH2 C-NH, NH NH CH, H,N-C CH, CH2 CH2 H,N-C H,N*-C- H. H. H,N-C C H H Aspartic acid (Asp or D) Arginine (Arg or R) Histidine Lysine (Lys or K) (His or H) Olon exchange chromatography Oan affnity matrix that binds to protein 3 only Oion exchange chromatography Oan affinity matrix that binds to protein 3 only OSDS-poly acrylamide gel electrophoresis Oan affinity matrix that binds to all 4 proteins equally Ogel filtration
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