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The following is the PCR reaction that is programmed in the thermocycler to amplify the cheek cell DNA: 1.95°C for 2 minutes 2. 95°C for 30 seconds 3.58.1°C for 30 seconds 4. 72°C for 45 seconds 5. Repeat steps 2-4 thirty (30) times 6. 72°C for 3 minutes 7. Hold at 16°C indefinitely Which steps represent the denaturation, annealing and extension steps?

The following is the PCR reaction that is programmed in the thermocycler to amplify the cheek cell DNA: 1.95°C for 2 minutes 2. 95°C for 30 seconds 3.58.1°C for 30 seconds 4. 72°C for 45 seconds 5. Repeat steps 2-4 thirty (30) times 6. 72°C for 3 minutes 7. Hold at 16°C indefinitely Which steps represent the denaturation, annealing and extension steps?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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The following is the PCR reaction that is programmed in the thermocycler to amplify the cheek cell DNA: 1. 95°C for 2 minutes 2. 95°C for 30 seconds 3. 58.1°C for 30 seconds 4. 72°C for 45 seconds 5. Repeat steps 2-4 thirty (30) times 6. 72°C for 3 minutes 7. Hold at 16°C indefinitely Which steps represent the denaturation, annealing and extension steps? O a. Step 4 = Denature; Step 6 = Anneal; Step 7 = Extend %3D O b. Step 3 = Denature; Step 4 = Anneal; Step 6 = Extend %3D !! C. Step 2 = Denature; Step 4 = Anneal; Step 6 = Extend %3D O d. Step 2 = Denature; Step 3 = Anneal; Step 4 = Extend
Transcribed Image Text:The following is the PCR reaction that is programmed in the thermocycler to amplify the cheek cell DNA: 1. 95°C for 2 minutes 2. 95°C for 30 seconds 3. 58.1°C for 30 seconds 4. 72°C for 45 seconds 5. Repeat steps 2-4 thirty (30) times 6. 72°C for 3 minutes 7. Hold at 16°C indefinitely Which steps represent the denaturation, annealing and extension steps? O a. Step 4 = Denature; Step 6 = Anneal; Step 7 = Extend %3D O b. Step 3 = Denature; Step 4 = Anneal; Step 6 = Extend %3D !! C. Step 2 = Denature; Step 4 = Anneal; Step 6 = Extend %3D O d. Step 2 = Denature; Step 3 = Anneal; Step 4 = Extend
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