9. The following sequence is a wild-type bacterial gene that encodes a short protein. The sequence given is from the point where transcription starts (called "+1") to the point where transcription ends. Transcription goes left to right. 5'-ACTTCGATATGTCTAAAATATCGATCGATCTGTGGGGCCTAGCTAGCTAACCAGAGACGCTACCG-3' 3'-TGAAGCTATACAGATTTTATAGCTAGCTAGACACCCCGGATCGATCGATTGGTCTCTGCGATGGC-5' (a) Which strand (the upper or the lower) is used as the template in transcription? (b) Write out the first 20 bases of the RNA transcribed from this wild-type gene. Make sure to label the 5' and 3' ends of your molecule. (c) Write out the amino acid sequence of any protein that is encoded by this wild-type gene. Make sure to label the N and C termini of your molecule.

9. The following sequence is a wild-type bacterial gene that encodes a short protein. The sequence given is from the point where transcription starts (called "+1") to the point where transcription ends. Transcription goes left to right. 5'-ACTTCGATATGTCTAAAATATCGATCGATCTGTGGGGCCTAGCTAGCTAACCAGAGACGCTACCG-3' 3'-TGAAGCTATACAGATTTTATAGCTAGCTAGACACCCCGGATCGATCGATTGGTCTCTGCGATGGC-5' (a) Which strand (the upper or the lower) is used as the template in transcription? (b) Write out the first 20 bases of the RNA transcribed from this wild-type gene. Make sure to label the 5' and 3' ends of your molecule. (c) Write out the amino acid sequence of any protein that is encoded by this wild-type gene. Make sure to label the N and C termini of your molecule.

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Similar questions

1. Given the DNA sequence below: 5’-ACATGTGTACAGGCTTTGTCTGAATGGCTT-3’ 3’-TGTACACATGTCCGAAACAGACTTACCGAA-5’ Translate the mRNA. (Using the 3-letter code for amino acids, write the primary structure of the peptide that will be produced.) 2. Given the DNA sequence below: 5’-ACATGTGTACAGGCTTTGTCTGAATGGCTT-3’ 3’-TGTACACATGTCCGAAACAGACTTACCGAA-5’ Translate the mRNA. (Using the 3-letter code for amino acids, write the primary structure of the peptide that will be produced.)
The template strand of a gene has the sequence 5'-ATGCCTAGCCTAGGACT-3'. What will the sequence of the transcript from this gene be? 5' AGUCCUAGGCUAGGCAU 3 5 ATGCCTAGCCTAGGACT 3' 5 AUGCCUAGCCUAGGACU 3' 5' MAUGCCUAGCCUAGGACUAAAAAAAAAA 3" 5' AGTCCTAGGCTAGGCAT 3" V.
You are studying the effect of various mutations in this gene. The AG sequence (3’splice site) that is within intron 2 [adjacent to Exon 3] has been changed to a CC. What would the resulting mRNA transcript look like? Draw a similar diagram as above and indicate the nucleotide lengths on the mRNA transcript.
17) Synthesis of the mRNA starts at the boxed A/T base pair indicated by the box and proceeds left to right on the sequence below. Transcribe and translate this bacterial gene. 5'-GGACCGCGGGGCAGGATTGCTCCGGGCTGTTTCATGACTIGICAGGTGGGATGACTTGGATGGAAAAGTAGAAGGTCATG-3 3'-CCTGGCGCCCCGTCCTAACGAGGCCCGACAAAGTACTGAACAGTCCACCCTACTGAACCTACCTTTTCATCTTCCAGTAC-5′ 1 -+--at - --+-- 80
Which of the following sequences would most likely be the site of the initiation of transcription? ТАТАААТА GCTAGCTA GGCGGCCC Impossible to tell
Consider the following sequence fragment of an mRNA. Which of the miRNAS below would be competent for gene silencing? 5'-AUGCAAGCAUUGGCCAAGCUU-3' 5'-AUGCAAGCAUUGGCCAAGCUU-3 5'-UACGUUCGUAACCGGUUCGAA-3' 5'-AAGCUUGGUUAAUGCUUGCAU-3' 5'-UUCGAACCAAUUACGAACGUA-3' 3'-UUCGAACCAAUUACGAACGUA-5 3'-AUGCAAGCAUUGGCCAAGCUU-5'
Given Sequence: 3’-TACGGTCCGGATTCGGTAGCTAGCATC-5’ Provide: Complementary Strand: Transcript Amino Acid Sequence 2.Given Sequence: 5’-GGGCATATGCCGTTTACCGGTTTGACTAAATAACCA-3’ Provide: Complementary Strand: Transcript Amino Acid Sequence 3.Given Sequence: 3’-AAC CAA TAC GTG AGG ATA CCA AGT AAC ACT CCC-5’ Provide: Complementary Strand: Direct Transcript: Transcript for Translation: Amino Acid Sequence: