3) Enzyme assay. When cyclin D1 activates Cdk4 and Cdk6, the activated Cdks phosphorylate retinoblastima protein (RB). Purified V was incubated with purified Cdk6, purified RB protein, and radiolabeled ATP. As a control, purified Cdk6, pur RB protein, and radiolabeled ATP were incubated without purified V. One hour lat the enzyme assay mixes were separated by PAGE. The PAGE gel exposed to X-ray film. The developed film is shown below. V/cdk6, What is the purpose of the control shown at left? What do the results on the right demonstrate? 525 words English (United States) O Focus “美 MacBook Pro cdk6

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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AaBbCcl AaBbCcD
AaBbCcDdl
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3) Enzyme assay. When cyclin D1 activates Cdk4 and Cdk6, the activated Cdks
phosphorylate retinoblastima protein (RB). Purified V was incubated with purified
Cdk6, purified RB protein, and radiolabeled ATP. As a control, purified Cdk6, purified
RB protein, and radiolabeled ATP were incubated without purified V. One hour later,
the enzyme assay mixes were separated by PAGE. The PAGE gel was
exposed to X-ray film. The developed film is shown below.
V/cdk6.
What is the purpose of the control shown at left?
What do the results on the right demonstrate?
299%
O Focus
525 words
English (United States)
MacBook Pro
cdk6
Transcribed Image Text:Mailings View O Tell me Layout References Review ert. AaBbCcl AaBbCcD AaBbCcDdl AaBbCcDdE - A A A al A Aa v Times New. 12 styles Dictate Sensitivity Heading 2 Heading 3 Heading 4 Heading 1 Pane A 2. A vA O 3) Enzyme assay. When cyclin D1 activates Cdk4 and Cdk6, the activated Cdks phosphorylate retinoblastima protein (RB). Purified V was incubated with purified Cdk6, purified RB protein, and radiolabeled ATP. As a control, purified Cdk6, purified RB protein, and radiolabeled ATP were incubated without purified V. One hour later, the enzyme assay mixes were separated by PAGE. The PAGE gel was exposed to X-ray film. The developed film is shown below. V/cdk6. What is the purpose of the control shown at left? What do the results on the right demonstrate? 299% O Focus 525 words English (United States) MacBook Pro cdk6
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