A mutant monkey was identified that showed resistance to a viral disease 'Kansah'. The kan gene, responsible for the disease resistance, was identified and cloned. A molecular technique revealed the presence of kan in human cells. Later these researchers isolated RNA from liver, kidney, gut and skin cells. The researchers prepared CDÑA using reverse transcriptase and then performed real-time PCR to measure the expression of the kan gene. The Ct values for samples from gut, kidney, liver and skin cells were 23, 21, 20 and 40, respectively. Which cells have the highest expression of kan? If a liver cell makes 2000 molecules of Kan protein, assuming equal translation efficiencies in all cell types how many molecules will you expect from a single kidney cell? gut cell?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
icon
Related questions
Question
Please help me with this question immediately within an hour need to submit ASAP
A mutant monkey was identified that showed
resistance to a viral disease 'Kansah'. The kan
gene, responsible for the disease resistance, was
identified and cloned. A molecular technique
revealed the presence of kan in human cells. Later
these researchers isolated RNA from liver, kidney,
gut and skin cells. The researchers prepared CDNA
using reverse transcriptase and then performed
real-time PCR to measure the expression of the
kan gene. The Ct values for samples from gut,
kidney, liver and skin cells were 23, 21, 20 and 40,
respectively.
Which cells have the highest expression of kan?
If a liver cell makes 2000 molecules of Kan protein,
assuming equal translation efficiencies in all cell
types how many molecules will you expect from a
single
kidney cell?
gut cell?
Transcribed Image Text:A mutant monkey was identified that showed resistance to a viral disease 'Kansah'. The kan gene, responsible for the disease resistance, was identified and cloned. A molecular technique revealed the presence of kan in human cells. Later these researchers isolated RNA from liver, kidney, gut and skin cells. The researchers prepared CDNA using reverse transcriptase and then performed real-time PCR to measure the expression of the kan gene. The Ct values for samples from gut, kidney, liver and skin cells were 23, 21, 20 and 40, respectively. Which cells have the highest expression of kan? If a liver cell makes 2000 molecules of Kan protein, assuming equal translation efficiencies in all cell types how many molecules will you expect from a single kidney cell? gut cell?
Expert Solution
steps

Step by step

Solved in 2 steps

Blurred answer
Knowledge Booster
Types of communication
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education