WACE: Sanger Sequencing Sequencing the DNA of a person who is heterozygous for an allele Sequencing Templates Allele 1 5' CCT GAG GAG 3' Allele 2 www 5' CCT GTG GAG 3' 1. What is the Primer location: A or B? 5' CCT GAG GAG 3' A www B 2. Draw the bands on the gel corresponding to the sequencing of allele 1. Indicate the 5' and 3' ends. 3. Draw any additional bands expected if allele 2 is also sequenced. Sequencing Gel 5 C T 3' G

Human Anatomy & Physiology (11th Edition)
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Author:Elaine N. Marieb, Katja N. Hoehn
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WACE: Sanger Sequencing
Sequencing the DNA of a person who is heterozygous for an allele
Sequencing Templates
Allele 1
5' CCT GAG GAG 3'
www
Allele 2
5' CCT GTG GAG 3'
1. What is the Primer location: A or B?
5' CCT GAG GAG 3'
wwww sanse
A
B
2. Draw the bands on the gel corresponding to the
sequencing of allele 1. Indicate the 5' and 3’ ends.
3. Draw any additional bands expected if allele 2 is also
sequenced.
A
Sequencing Gel
5'
C
T G
3'
Transcribed Image Text:WACE: Sanger Sequencing Sequencing the DNA of a person who is heterozygous for an allele Sequencing Templates Allele 1 5' CCT GAG GAG 3' www Allele 2 5' CCT GTG GAG 3' 1. What is the Primer location: A or B? 5' CCT GAG GAG 3' wwww sanse A B 2. Draw the bands on the gel corresponding to the sequencing of allele 1. Indicate the 5' and 3’ ends. 3. Draw any additional bands expected if allele 2 is also sequenced. A Sequencing Gel 5' C T G 3'
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