None You extracted DNA from a wildtype strain of E. coli and used the DNA to transform astrain that was arg gal lys and gly. You select for transformation of the individualloci (initial selection) and then determine the co-transformation frequency for theother loci, plotted in the table below:Initial selectionCo-transformedargt galt lys glyarg+0% 60% 15%gal+0%0% 10%lys+60% 0%0%gly+15% 10% 0%Based on these results, which of the following options is the mostlikely order of the genes?lys gal arg glylys gly gal argarg lys gly galgly gal arg lysgal gly arg lysgly arg gal lys

Answered: You extracted DNA from a wildtype…

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 20QP: Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant...

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Five different HFR strains were isolated from a single F+ strain of E. coli. Timed matings showed that they transferred the following loci during the first hour of mating. The earliest loci are on the left, the slowest on the right for each order. Map these loci to a circle. strain early →→late 1. QZ ARL What markers are between B and X (shortest route)? SNWT O EMQ 3. 5. RBLE XAR BEMQZ EBLRA ZSNWT strain early →late 2. 4. TXARL XTWNS
In a transformation experiment, donor DNA was obtained from a prototroph bacterial strain (x* y z'), and the recipient was a triple auxotroph (x y z ). The following transformant classes were obtained: x* y z xy'z 160 130 190 170 x'y 3 xy x'y 5 What general conclusions can you draw about the linkage relationships among the three genes?
The following two strains of E. coli are crossed with each other: Hfr pan* thi* ala* and F¯pan¯ thi¯ ala¯ It was shown that the pan marker entered last in interrupted conjugation experiments. By spreading the bacteria from these experiments on different selection media, the following results (in number of colonies) were obtained: MM + Gluc IM + Gluc + ala MM + Gluc + thi MM + Gluc + thi + ala 280 281 286 339 Give the number of each phenotypic class and justify your answer. Determine the order of the genes. What are the genetic distances between the different loci? Abbreviations: Gluc: glucose; Ala: alanine; Thi: thiamine; Pan: pantothenic acid.
Determine the map distances between the genes.
Which RE can be used to distinguish bacterial strain 1 from strain 2 based on a sample DNA fragment from each strain (as shown below)? STRAIN 1 ~712 bp STRAIN 2 ~712 bp Restriction enzymes: O BglIII, Ndel, and Ncol O Ncol O Ndel OBgIIII CATATGCCATGGAGATCT GTATACGGTACCTCTAGA ~231 bp CACATGCCATGGAGATCT GTGTACGGTACCTCTAGA 231 bp BglIII Ncol AGATCT CCATGG TCTAGA GGTACC Nde1 CATATG GTATAC
Streptococcus pneumoniae cells of genotype str s mtl - are transformed by donor DNA of genotype strr mtl+ and (in a separate experiment) by a mixture of two DNAs with genotypes strr mtl - and str s mtl+. The accompanying table shows the results.a. What does the first row of the table tell you? Why? b. What does the second row of the table tell you? Why?
DNA from a strain of Bacillus subtilis with genotype a+ b+ c+ d+ e+ is used to transform a strain with genotype a– b– c– d– e–. Pairs of genes are checked for co-transformation and results shown below are obtained. Based on these results, what is the order of the genes on the bacterial chromosome? please show how you worked out the order by diagraming, thank you! Pair of genes Co-transformation a+ and b+ no a+ and c+ no a+ and d+ yes a+ and e+ yes b+ and c+ yes b+ and d+ no b+ and e+ no c+ and d+ no c+ and e+ yes d+ and e+ no
In E. coli, four Hfr strains donate the following markers,shown in the order donated:Strain 1: M Z X W CStrain 2: L A N C WStrain 3: A L B R UStrain 4: Z M U R BAll these Hfr strains are derived from the same F+ strain.What is the order of these markers on the circularchromosome of the original F+?
In the experiment of Figure shown, Lederberg and Tatum could notdiscern whether met+ bio+ genetic material was transferred to themet− bio− thr+ leu+ thi+ strain or if thr+ leu+ thi+ genetic materialwas transferred to the met+ bio+ thr− leu− thi− strain. Let’s supposethat one strain is streptomycin-resistant (say, met+ bio+ thr− leu−thi−) and the other strain is sensitive to streptomycin. Describe anexperiment that could determine whether the met+ bio+ geneticmaterial was transferred to the met − bio− thr+ leu+ thi+ strain orthe thr+ leu+ thi+ genetic material was transferred to the met+ bio+thr− leu− thi− strain. bio+ thr− leu− thi− strain.
The genomes of two E. coli strains are compared in Figure14-19. Would you expect any third strain to contain moreof the blue, tan, or red regions shown in Figure 14-19?Explain.
Determine the coefficient of coincidence and the interference
DNA from a strain of Bacillus subtilis with the genotype trp+ tyr+ was used to transform a recipient strain with the genotype trp− tyr−. The following numbers of transformed cells were recovered: Genotype Number of transformed cells trp+ tyr− 154 trp− tyr+ 312 trp+ tyr+ 354 What do these results suggest about the linkage of the trp and tyr genes?

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