In and out M 3. What are restriction enzymes used for in nature? are used by backsta ta bock ria to cat yo Restriction enzymes are used the DNA. 4. Look at the Jellyfish Glo gene. The start (TAC) and stop (ACT) DNA sequences of the gene are marked by black boxes. What process are they the start and stop of? 5. Why did we make sure to include these start and stop DNA sequences for the Jellyfish Glo gene in our cut segment? 6. If we want to now produce a lot of this Jellyfish Glo protein, what do we have to do now with the transformed bacteria to get a lot of protein from them? procedure. 7. Identify the structure in each section of the diagram and explain what its role is in this whole

In and out M 3. What are restriction enzymes used for in nature? are used by backsta ta bock ria to cat yo Restriction enzymes are used the DNA. 4. Look at the Jellyfish Glo gene. The start (TAC) and stop (ACT) DNA sequences of the gene are marked by black boxes. What process are they the start and stop of? 5. Why did we make sure to include these start and stop DNA sequences for the Jellyfish Glo gene in our cut segment? 6. If we want to now produce a lot of this Jellyfish Glo protein, what do we have to do now with the transformed bacteria to get a lot of protein from them? procedure. 7. Identify the structure in each section of the diagram and explain what its role is in this whole

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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5. A scientist was studying a fragment of eukaryotic DNA that she suspected was involved in a disease process. She isolated the fragment from the genomic DNA and then labeled the ends of the DNA with radioactive tags. This radioactively labeled fragment was then subjected to 3 restriction enzyme digest treatments: EcoRI single digest, BamHI single digest and ECORI/BamHI double digest. The results are shown on the following table. The asterisk (*) indicated fragments containing the radioactive label. Restriction Enzyme Fragment Size (bp) |400, 200*, 100*, 50 350*, 250, 150* 250, 200*, 10o*, 50 ВатHI EcoRI +BamHI Draw the restriction map for this DNA fragment.
7. e is not the write answer
How does that length compare to that of restriction enzymes? Implications? 2. can you conceive of any potential applications of CRISPR technology?
DNA Restriction, Electrophoresis 1. What are restriction enzymes? 2. How do restriction enzymes function? 3. Why are restriction enzymes important in biochemistry labs? 4. What is DNA Gel Electrophoresis? 5. How does DNA Gel Electrophoresis function? 6. Why is DNA Gel Electrophoresis important in biochemistry labs?
A 4. You are given three different substances that are known mutagens. Using a variety of techniques, you analyze the results of exposure to these substances. Your findings are shown in the table below. Using this data, link each of the three substances with one of the following molecular properties and explain your choice. B C I. Substance II. III. One mutagen that looks like cytosine but forms a covalent bond with guanine One mutagen that can bind with either adenine or guanine A molecule that can insert itself between two base pairs in a DNA strand Result of exposure Increases the number of mutants that produce mRNA with AGA codons instead of AAA codons Significantly fewer viable colonies Increase in mutants containing frameshift mutations
4. How is a randomized DNA library created? MARK ALL THAT APPLY. the use of Mn2+ rather than Mg2+ by DNA Polymerase PCR with dideoxynucleotides Mutagenic PCR Lots of books Add more dNTPs than normal to a PCR reaction
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4. A linear piece of DNA has the following EcoRI restriction sites. EcoRI site 1 2 kb 4 kb EcoRI site 2 5 kb a. This piece of DNA is cut by EcoRI, the resulting fragments are separated by gel electrophoresis, and the gel is stained with ethidium bromide. Draw a picture of the bands that will appear on the gel.
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y Cours S DIOE The following DNA fragment shows where a number of restriction endonucleases cut sites occur within the fragment. The numbers below indicate the distance between those cut sites in base pairs (bp). Kpn I Sall Eco RI Pst Bam HI Xho Bam HI Pst i Eco RI Kon I 100 200 300 400 500 B00 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900 2000 How many total restriction fragments will result if you complete digest the DNA fragment above with Sal I? cross out a. 1 cross out b. 2 cross out O c. 3 cross out d. 4 cross out e. 5 cross out O f. 6 cross out g. 7 cross out Oh. 8 The following DNA fragment shows where a number of restriction endonucleases cut sites occur within the fragment. The numbers below indicate the distance between those cut sites in base pairs (bp). t of Sal Eco RI Pat Xhol Bam HI Psti Eco RI Koni Kpn i Bam H
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