In and out M 3. What are restriction enzymes used for in nature? are used by backsta ta bock ria to cat yo Restriction enzymes are used the DNA. 4. Look at the Jellyfish Glo gene. The start (TAC) and stop (ACT) DNA sequences of the gene are marked by black boxes. What process are they the start and stop of? 5. Why did we make sure to include these start and stop DNA sequences for the Jellyfish Glo gene in our cut segment? 6. If we want to now produce a lot of this Jellyfish Glo protein, what do we have to do now with the transformed bacteria to get a lot of protein from them? procedure. 7. Identify the structure in each section of the diagram and explain what its role is in this whole

In and out M 3. What are restriction enzymes used for in nature? are used by backsta ta bock ria to cat yo Restriction enzymes are used the DNA. 4. Look at the Jellyfish Glo gene. The start (TAC) and stop (ACT) DNA sequences of the gene are marked by black boxes. What process are they the start and stop of? 5. Why did we make sure to include these start and stop DNA sequences for the Jellyfish Glo gene in our cut segment? 6. If we want to now produce a lot of this Jellyfish Glo protein, what do we have to do now with the transformed bacteria to get a lot of protein from them? procedure. 7. Identify the structure in each section of the diagram and explain what its role is in this whole

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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3. A linear DNA fragment is cleaved with one, two or three restriction enzymes to yield fragments as follows: Enzyme(s) Fragments (kb) HindIII 2.5, 5.0 Smal 2.0, 5.5 HindIII + Smal 2.5, 3.0, 2.0 HindIII+ Smal+ EcoRI 1.5, 2.0, 2.5 Draw the restriction map of the original DNA fragment, indicating the positions of all restriction sites.
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all parts are together
23. Important elements of a directed evolution experiment (“evolution in a test tube”) for an ATP-binding aptamer include: MARK ALL THAT APPLY. Group of answer choices Mutagenic PCR DpnI restriction enzyme Randomized DNA pool ATP affinity column
Please answer fast 1. Genome annotation refers to all of the following except a. assigning a unique identifier given to a DNA or protein sequence recordb. locating genes in sequencesc. adding known functions to the sequenced. assigning the sequence to a functional categorye. All of the above are correct 2. Which of the following databases contain curated annotations about human genes? a. GENCODEb. RefSeq c. UniProtKBd. All of the above 3. Which database covers a wider range of functional definitions and therefore would bea better choice for exploring the genome for new genes? a. GENCODEb. RefSeqc. UniProtKBd. All of the above 4. AKT1 is the official ___________________ for a gene. a. gene IDb. namec. symbold. all of the above 5. Which BLAST (Basic Local Alignment Search Tool) variant would you select toconduct a translated nucleotide query? a. BLASTnb. BLASTpc. BLASTxd. TBLASTne. TBLASTx
Briefly, be able to define each of these AND, where relevant, tell what do they do, and which process(es) are they involved in (for example, replication or translation or transcription or splicing etc.) * means can you draw it (stick figure)?
9
Incorrect 1 A Help Center? It can sequence a whole genome in one huge strand Each nucleotide has a different marker attached Question 11 LO62 Explain how SANGER sequencing works Order the steps that are needed for gene sequencing through Sanger technology: Amplify DNA Cut DNA into small pieces Add DNA to be sequenced + DNA polymerase + dNTPs + ddNTPs into different test tubes Run resulting sequences through gel electrophoresis 3 111 0/1 pts 0 / 1 pts
5. A scientist was studying a fragment of eukaryotic DNA that she suspected was involved in a disease process. She isolated the fragment from the genomic DNA and then labeled the ends of the DNA with radioactive tags. This radioactively labeled fragment was then subjected to 3 restriction enzyme digest treatments: EcoRI single digest, BamHI single digest and ECORI/BamHI double digest. The results are shown on the following table. The asterisk (*) indicated fragments containing the radioactive label. Restriction Enzyme Fragment Size (bp) |400, 200*, 100*, 50 350*, 250, 150* 250, 200*, 10o*, 50 ВатHI EcoRI +BamHI Draw the restriction map for this DNA fragment.
7. e is not the write answer
How does that length compare to that of restriction enzymes? Implications? 2. can you conceive of any potential applications of CRISPR technology?