The short DNA shown below is to be sequenced. Using your knowledge of how the Sanger method works, in the gel diagram, draw in the bands that will appear when DNA polymerase is added to the reaction along with the four different nucleotide mixtures indicated. Note that some of these mixtures are not what would normally be used in a sequencing reaction. Dideoxwnucleotides (ddNTPs) are added in relatively small amounts. The asterisk represents a radioactive label. *5' --- 3'-OH 3' --- ACGACGCAGGACATTAGAC-5' Nucleotide mixtures: A. DATP, dTTP, ACTP, ¿GTP, ddTIR (given) B. DATR, dTTP, ¿CTP, AGTP, ddATP C. dTTP, dGTP, dCTP. ddCTP, ddATR D. DATP, ¿CTP, dTTP, ddGTP А В С D |LL ||

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The short DNA shown below is to be sequenced. Using your knowledge of how the Sanger method
works, in the gel diagram, draw in the bands that will appear when DNA polymerase is added to the
reaction along with the four different nucleotide mixtures indicated. Note that some of these mixtures are
not what would normally be used in a sequencing reaction. Dideoxynucleatides (ddNTPs) are added in
relatively small amounts. The asterisk represents a radioactive label.
*5'
- 3'-ОН
3' –
-- ACGACGCAGGACATTAGAC-5'
Nucleotide mixtures:
A. DATP, DTTP, dCTP, DGTP, ddTTP (given)
B. DATP, ATTP, dCTP, AGTP, ddATE
C. dTTP, dGTP. ACTP, ddCTP, ddATP
D. DATP, dCTP, dTTP, ddGTP
A
в с D
| || ||
Transcribed Image Text:The short DNA shown below is to be sequenced. Using your knowledge of how the Sanger method works, in the gel diagram, draw in the bands that will appear when DNA polymerase is added to the reaction along with the four different nucleotide mixtures indicated. Note that some of these mixtures are not what would normally be used in a sequencing reaction. Dideoxynucleatides (ddNTPs) are added in relatively small amounts. The asterisk represents a radioactive label. *5' - 3'-ОН 3' – -- ACGACGCAGGACATTAGAC-5' Nucleotide mixtures: A. DATP, DTTP, dCTP, DGTP, ddTTP (given) B. DATP, ATTP, dCTP, AGTP, ddATE C. dTTP, dGTP. ACTP, ddCTP, ddATP D. DATP, dCTP, dTTP, ddGTP A в с D | || ||
Expert Solution
Step 1

This scenario based on DNA sequencing. DNA sequencing is the process of determining the order of nucleotides in DNA. DNA sequencing in various type such as

  • Maxam and Gilbert method.
  • Chain termination method. or Sanger sequencing,
  • semiautomated method.
  • automated method.
  • Pyrosequencing.
  • The whole-genome shotgun sequencing method.
  • Clone by the clone sequencing method.
  • Next-generation sequencing method. etc.

Sanger sequencing is a method for determining the nucleotide sequence of DNA, that involves electrophoresis and is based on the random incorporation of chain-terminating dideoxynucleotides.

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