The goal of the Knockout Mouse Project is to generate a set of ES cell lines, each with a knockout mutation in a single gene, that collectively contains amutation in every gene in the mouse genome.a. Do you think that it will be possible, for everygene, to generate a heterozygous knockout ES cellline? Explain.b. Do you think that for every heterozygous knockoutES cell line, it will be possible to generate a heterozygous knockout mouse? Explain.c. In fact, investigators failed in their attempts to generate ES cell knockouts for the Fam gene describedin Problem 5. How could these researchers use EScell technology to determine whether Fam is required for eye development in the mouse? Diagrama construct that the researchers could introduceinto ES cells to explore this issue.d. Describe various outcomes that might be obtainedfrom the experiment in part (c) and what you couldconclude in each case.
The goal of the Knockout Mouse Project is to generate a set of ES cell lines, each with a knockout mutation in a single gene, that collectively contains a
mutation in every gene in the mouse genome.
a. Do you think that it will be possible, for every
gene, to generate a heterozygous knockout ES cell
line? Explain.b. Do you think that for every heterozygous knockout
ES cell line, it will be possible to generate a heterozygous knockout mouse? Explain.
c. In fact, investigators failed in their attempts to generate ES cell knockouts for the Fam gene described
in Problem 5. How could these researchers use ES
cell technology to determine whether Fam is required for eye development in the mouse? Diagram
a construct that the researchers could introduce
into ES cells to explore this issue.
d. Describe various outcomes that might be obtained
from the experiment in part (c) and what you could
conclude in each case.
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