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The basis for the 1-pot DNA sequencing method (discussed in the lecture) is use of ddNTPs (2',3'-dideoxy NTPS), with each one linked to a different fluorophore that emits light at different wavelengths. (e.g., ddATP linked to a molecule that fluoresces red light, ddTTP linked to a blue fluorophore, etc.) True O False

The basis for the 1-pot DNA sequencing method (discussed in the lecture) is use of ddNTPs (2',3'-dideoxy NTPS), with each one linked to a different fluorophore that emits light at different wavelengths. (e.g., ddATP linked to a molecule that fluoresces red light, ddTTP linked to a blue fluorophore, etc.) True O False

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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The basis for the 1-pot DNA sequencing method (discussed in the lecture) is use of ddNTPs (2',3'-dideoxy NTPS), with each one linked to a different fluorophore that emits light at different wavelengths. (e.g., ddATP linked to a molecule that fluoresces red light, ddTTP linked to a blue fluorophore, etc.) True O False
Transcribed Image Text:The basis for the 1-pot DNA sequencing method (discussed in the lecture) is use of ddNTPs (2',3'-dideoxy NTPS), with each one linked to a different fluorophore that emits light at different wavelengths. (e.g., ddATP linked to a molecule that fluoresces red light, ddTTP linked to a blue fluorophore, etc.) True O False
A ribonucleotide consists of a nucleobase (e.g. guanine, uracil) 33 Minutes, g connected to a ribose that is phosphorylated at the 5' position. O True ne O False
Transcribed Image Text:A ribonucleotide consists of a nucleobase (e.g. guanine, uracil) 33 Minutes, g connected to a ribose that is phosphorylated at the 5' position. O True ne O False
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