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The amplified target DNA is now ready to be cloned into a plasmid. The next step is to perform a restriction enzyme digest on the amplified target DNA and plasmid. The plasmid map is shown below. 400 bp Hae III 380 bp Bam HI Eco RI 150 bp Hind II Plasmid DNA 2000 bp 250 bp 500 bp Eco RI 320 bp Eco RV a. Look up the restriction enzyme and indicate whether the restriction enzyme will produce a blunt or staggered cut. (1 mark) Note: Type either Blunt or Staggered Restriction enzyme Eco RI Type of cut (Blunt vs Staggered) Staggered b. Based on the plasmid map, list the size of fragments produced when the following restriction enzymes are used. Note: to answer correctly you must order the fragments from smallest to largest and separate each with a comma. There should be no spaces between numbers and commas. e.g. 400,500,530 Restriction Enzyme Eco RI and Hind II Fragment size 300, 700 200, 800 Hae III, Eco RI and Bam HI 100, 200, 700 Eco RV and Bam HI

The amplified target DNA is now ready to be cloned into a plasmid. The next step is to perform a restriction enzyme digest on the amplified target DNA and plasmid. The plasmid map is shown below. 400 bp Hae III 380 bp Bam HI Eco RI 150 bp Hind II Plasmid DNA 2000 bp 250 bp 500 bp Eco RI 320 bp Eco RV a. Look up the restriction enzyme and indicate whether the restriction enzyme will produce a blunt or staggered cut. (1 mark) Note: Type either Blunt or Staggered Restriction enzyme Eco RI Type of cut (Blunt vs Staggered) Staggered b. Based on the plasmid map, list the size of fragments produced when the following restriction enzymes are used. Note: to answer correctly you must order the fragments from smallest to largest and separate each with a comma. There should be no spaces between numbers and commas. e.g. 400,500,530 Restriction Enzyme Eco RI and Hind II Fragment size 300, 700 200, 800 Hae III, Eco RI and Bam HI 100, 200, 700 Eco RV and Bam HI

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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The amplified target DNA is now ready to be cloned into a plasmid. The next step is to perform a restriction
enzyme digest on the amplified target DNA and plasmid. The plasmid map is shown below.

 

 Look up the restriction enzyme and indicate whether the restriction enzyme will produce a blunt
or staggered cut. (1 mark)
Note: Type either Blunt or Staggered (refer to the image)

 

b. Based on the plasmid map, list the size of fragments produced when the following restriction enzymes
are used.
Note: to answer correctly you must order the fragments from smallest to largest and separate each with a
comma. There should be no spaces between numbers and commas. e.g. 400,500,530 (refer to the image) 

 

Note: Please refer to the image for everything related!. 
check answers for part b). Do not use AI i need authentic answers 

The amplified target DNA is now ready to be cloned into a plasmid. The next step is to perform a restriction enzyme digest on the amplified target DNA and plasmid. The plasmid map is shown below. 400 bp Hae III 380 bp Bam HI Eco RI 150 bp Hind II Plasmid DNA 2000 bp 250 bp 500 bp Eco RI 320 bp Eco RV a. Look up the restriction enzyme and indicate whether the restriction enzyme will produce a blunt or staggered cut. (1 mark) Note: Type either Blunt or Staggered Restriction enzyme Eco RI Type of cut (Blunt vs Staggered) Staggered b. Based on the plasmid map, list the size of fragments produced when the following restriction enzymes are used. Note: to answer correctly you must order the fragments from smallest to largest and separate each with a comma. There should be no spaces between numbers and commas. e.g. 400,500,530 Restriction Enzyme Eco RI and Hind II Fragment size 300, 700 200, 800 Hae III, Eco RI and Bam HI 100, 200, 700 Eco RV and Bam HI
Transcribed Image Text:The amplified target DNA is now ready to be cloned into a plasmid. The next step is to perform a restriction enzyme digest on the amplified target DNA and plasmid. The plasmid map is shown below. 400 bp Hae III 380 bp Bam HI Eco RI 150 bp Hind II Plasmid DNA 2000 bp 250 bp 500 bp Eco RI 320 bp Eco RV a. Look up the restriction enzyme and indicate whether the restriction enzyme will produce a blunt or staggered cut. (1 mark) Note: Type either Blunt or Staggered Restriction enzyme Eco RI Type of cut (Blunt vs Staggered) Staggered b. Based on the plasmid map, list the size of fragments produced when the following restriction enzymes are used. Note: to answer correctly you must order the fragments from smallest to largest and separate each with a comma. There should be no spaces between numbers and commas. e.g. 400,500,530 Restriction Enzyme Eco RI and Hind II Fragment size 300, 700 200, 800 Hae III, Eco RI and Bam HI 100, 200, 700 Eco RV and Bam HI
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