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QUESTION 20 Careful TEM studies suggested that histone octamers get removed during DNA replication. This raised a question regarding what happens to the histone octamers after they get removed. Do the histone octamers get disassembled after removal, or do they remain intact and get added back onto the DNA without disassembly. The figure below outlines an experiment that researchers performed to address this question. In a single sentence, describe the conclusion that can be drawn from this experiment. Experiment Question: What happens to histones in eukaryotic DNA replication? Methods 1 Grow cells for several generations in a medium that contains amino acids labeled with a heavy isotope. 2 Transfer the cells to a medium that contains amino acids labeled with a light isotope. Results Change medium Replication Isolate octamers 3 Isolate histone octamers before and after replication... Spin 4 ...and subject them to density-L gradient centrifugation. 5 Newly synthesized octamers are less dense and thus will be higher in the tube. 6 Old octamers are dense and will move toward the bottom of the tube. Isolate octamers Spin

QUESTION 20 Careful TEM studies suggested that histone octamers get removed during DNA replication. This raised a question regarding what happens to the histone octamers after they get removed. Do the histone octamers get disassembled after removal, or do they remain intact and get added back onto the DNA without disassembly. The figure below outlines an experiment that researchers performed to address this question. In a single sentence, describe the conclusion that can be drawn from this experiment. Experiment Question: What happens to histones in eukaryotic DNA replication? Methods 1 Grow cells for several generations in a medium that contains amino acids labeled with a heavy isotope. 2 Transfer the cells to a medium that contains amino acids labeled with a light isotope. Results Change medium Replication Isolate octamers 3 Isolate histone octamers before and after replication... Spin 4 ...and subject them to density-L gradient centrifugation. 5 Newly synthesized octamers are less dense and thus will be higher in the tube. 6 Old octamers are dense and will move toward the bottom of the tube. Isolate octamers Spin

Concepts of Biology
Concepts of Biology
1st Edition
ISBN:9781938168116
Author:Samantha Fowler, Rebecca Roush, James Wise
Publisher:Samantha Fowler, Rebecca Roush, James Wise
Chapter9: Molecular Biology
Section: Chapter Questions
Problem 1ACQ: Figure 9.10 You isolate a cell strain in which the joining together of Okazaki fragments is impaired...
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QUESTION 20 Careful TEM studies suggested that histone octamers get removed during DNA replication. This raised a question regarding what happens to the histone octamers after they get removed. Do the histone octamers get disassembled after removal, or do they remain intact and get added back onto the DNA without disassembly. The figure below outlines an experiment that researchers performed to address this question. In a single sentence, describe the conclusion that can be drawn from this experiment. Experiment Question: What happens to histones in eukaryotic DNA replication? Methods 1 Grow cells for several generations in a medium that contains amino acids labeled with a heavy isotope. 2 Transfer the cells to a medium that contains amino acids labeled with a light isotope. Results Change medium Replication Isolate octamers 3 Isolate histone octamers before and after replication... Spin 4 ...and subject them to density-L gradient centrifugation. 5 Newly synthesized octamers are less dense and thus will be higher in the tube. 6 Old octamers are dense and will move toward the bottom of the tube. Isolate octamers Spin
Transcribed Image Text:QUESTION 20 Careful TEM studies suggested that histone octamers get removed during DNA replication. This raised a question regarding what happens to the histone octamers after they get removed. Do the histone octamers get disassembled after removal, or do they remain intact and get added back onto the DNA without disassembly. The figure below outlines an experiment that researchers performed to address this question. In a single sentence, describe the conclusion that can be drawn from this experiment. Experiment Question: What happens to histones in eukaryotic DNA replication? Methods 1 Grow cells for several generations in a medium that contains amino acids labeled with a heavy isotope. 2 Transfer the cells to a medium that contains amino acids labeled with a light isotope. Results Change medium Replication Isolate octamers 3 Isolate histone octamers before and after replication... Spin 4 ...and subject them to density-L gradient centrifugation. 5 Newly synthesized octamers are less dense and thus will be higher in the tube. 6 Old octamers are dense and will move toward the bottom of the tube. Isolate octamers Spin
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