ques 1 . A small DNA molecule was cleaved with several different restriction nucleases, and the size of each fragment was determined by gel electrophoresis. The following data were obtained: Enzyme Fragment size (kb). EcoRI 1.3, 1.3 Hpall 2.6 HindIII 2.6 EcoRI+Hpall 1.3, 0.8, 0.5 EcoRI + HindIII 0.6, 0.7, 1.3 a. Is the original molecule linear or circular? b. Draw a map of restriction sites, showing distances between sites, that is consistent with the data presented.
ques 1 . A small DNA molecule was cleaved with several different restriction nucleases, and the size of each fragment was determined by gel electrophoresis. The following data were obtained:
Enzyme Fragment size (kb).
EcoRI 1.3, 1.3
Hpall 2.6
HindIII 2.6
EcoRI+Hpall 1.3, 0.8, 0.5
EcoRI + HindIII 0.6, 0.7, 1.3
a. Is the original molecule linear or circular?
b. Draw a map of restriction sites, showing distances between sites, that is consistent with the data presented.
ques 2. You have double-stranded DNA that you radioactively label at the 5' ends. Digestion of this molecule with either EcoRI or BamHI yields the following fragments. The numbers are in kilobases (kb) and an asterisk Indicates the fragments that are unlabled
EcoRI: 2.8, 4:6, 6.2*, 7.4, 8.0*
BamHI: 6.0%, 10.0", 13.0
IF unlabeled DNA is digested with both enzymes simultaneously, the fragments 1.0, 2.0, 2.8, 3.6, 6.0, 6.2,7.4. What is the restriction map for the two enzymes?
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