1. A 2 kb fragment of DNA was cut by EcoRI and BamHI and then analyzed by gel electrophoresis. The following fragment sizes were produced: EcoRI: 900, 700, 400 BamHI: 1100, 900 In order to map the location of each restriction site, each fragment was then digested with the other two restriction enzymes. The following was obtained: ECORI BamHI Original Fragment 900 700 400 1100 900 EcoRI-Treated 700, 400 700, 200 BamHI-Treated 700, 200 700 400 Based on the fragment size analysis performed above, construct the map of restriction sites for this 2 kb fragment of DNA.
1. A 2 kb fragment of DNA was cut by EcoRI and BamHI and then analyzed by gel electrophoresis. The following fragment sizes were produced: EcoRI: 900, 700, 400 BamHI: 1100, 900 In order to map the location of each restriction site, each fragment was then digested with the other two restriction enzymes. The following was obtained: ECORI BamHI Original Fragment 900 700 400 1100 900 EcoRI-Treated 700, 400 700, 200 BamHI-Treated 700, 200 700 400 Based on the fragment size analysis performed above, construct the map of restriction sites for this 2 kb fragment of DNA.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Question
Can you help with q 1 please
Transcribed Image Text:1.
A 2 kb fragment of DNA was cut by EcoRI and BamHI and then analyzed by gel electrophoresis. The following
fragment sizes were produced:
EcoRI: 900, 700, 400
BamHI: 1100, 900
In order to map the location of each restriction site, each fragment was then digested with the other two restriction
enzymes. The following was obtained:
ECORI
BamHI
Original Fragment
900
700
400
1100
900
EcoRI-Treated
700, 400
700, 200
BamHI-Treated
700, 200
700
400
Based on the fragment size analysis performed above, construct the map of restriction sites for this 2 kb
fragment of DNA.
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 2 steps with 1 images
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education