Q1. Bioluminescence is emitted by the marine bacterium Vibrio fischeri in response to the concentration of a chemical signal. The luxCDABEG genes form part of an operon that encodes all of the structural components necessary for light production. At high cell density, the concentration of the inducer increases and can bind to LuxR protein which then activates transcription of the operon. What kind of operon is the Lux operon? [Positive Inducible, Positive Repressible, Negative Inducible, Negative Repressible?] Q2. A population of bacteria has a Lux operon that produces bioluminesence via an autoinducer that activates a transcription factor. You grow a flask of cells that have a loss-of-function mutation in the gene that encodes the enzyme that produces an autoinducer. What is the predicted bioluminesence in this population at high and low density?
Q1. Bioluminescence is emitted by the marine bacterium Vibrio fischeri in response to the concentration of a chemical signal. The luxCDABEG genes form part of an operon that encodes all of the structural components necessary for light production. At high cell density, the concentration of the inducer increases and can bind to LuxR protein which then activates transcription of the operon.
What kind of operon is the Lux operon? [Positive Inducible, Positive Repressible, Negative Inducible, Negative Repressible?]
Q2. A population of bacteria has a Lux operon that produces bioluminesence via an autoinducer that activates a transcription factor. You grow a flask of cells that have a loss-of-function mutation in the gene that encodes the enzyme that produces an autoinducer.
What is the predicted bioluminesence in this population at high and low density?
An operon is a cluster of genes under a single promoter. It consists of structural genes(coding region), regulatory genes (activator or repressor) and regulatory regions(promoter and operator).
An inducible operon is an operon which is turned ON in response to regulatory molecules such as an inducer. For example- Lac operon.
A repressible operon is an operon which is switched OFF in response to a regulatory molecule such as a repressor. For example- Tryptophan operon.
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