Kha Vu Danels Include: 8DX : Safehy Jor bromie tnto lab repart! Name Section date sheet MAPPING PRACTICE #1 Below is a restriction map for the plasmid PGEN 101 (total length = 20 Kb). Using this map as a guide, give the number of restriction fraqments along with their associated lengths that would result from digesting PGEN 101 with the restriction enzymes EcoRI, BamHI and a combination of ECORI and BamHI. BamHI 3.2 Kb 1.7 Kb EcoRI BamHI PGEN 101 8.7 Kb 5.5 Kb .9 Kb EcoRI ECORI DIGESTION PERFORMED SIZES OF FRAGMENTS OBTAINED 10.4 kb , 0.9kb, 8.7 Kb EcoRI 3.2 Kb, 16. 8kb BamHI EcoRI + BamHI

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Kha Vu Danels
Include: 8DX : Safehy Jor bromie tnto lab repart!
Name
Section
date sheet
MAPPING PRACTICE #1
Below is a restriction map for the plasmid PGEN 101 (total length = 20 Kb). Using this map as a
guide, give the number of restriction fraqments along with their associated lengths that would result
from digesting PGEN 101 with the restriction enzymes EcoRI, BamHI and a combination of ECORI and
BamHI.
BamHI
3.2 Kb
1.7 Kb
EcoRI
BamHI
PGEN 101
8.7 Kb
5.5 Kb
.9 Kb
EcoRI ECORI
DIGESTION PERFORMED
SIZES OF FRAGMENTS OBTAINED
10.4 kb , 0.9kb, 8.7 Kb
EcoRI
3.2 Kb, 16. 8kb
BamHI
EcoRI + BamHI
Transcribed Image Text:Kha Vu Danels Include: 8DX : Safehy Jor bromie tnto lab repart! Name Section date sheet MAPPING PRACTICE #1 Below is a restriction map for the plasmid PGEN 101 (total length = 20 Kb). Using this map as a guide, give the number of restriction fraqments along with their associated lengths that would result from digesting PGEN 101 with the restriction enzymes EcoRI, BamHI and a combination of ECORI and BamHI. BamHI 3.2 Kb 1.7 Kb EcoRI BamHI PGEN 101 8.7 Kb 5.5 Kb .9 Kb EcoRI ECORI DIGESTION PERFORMED SIZES OF FRAGMENTS OBTAINED 10.4 kb , 0.9kb, 8.7 Kb EcoRI 3.2 Kb, 16. 8kb BamHI EcoRI + BamHI
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