Example: A plant was discovered in a spring. To study the activity of the plant, 5.00g of the plant was finely grounded and mixed with an extraction buffer to make up a total of 1.00 mL crude plant extract. 19.00 ml of water was added to the plant extract. To determine the protein concentration of the extract, bovine serum albumin stock solution with a concentration of 200 μg mL -1 was used and mixtures with the following compositions and absorbances values were prepared (picture below). A 200 μg aliquot of the diluted plant extract was added with 300 μl of water and was subjected to Bradford assay. What is the protein concentration, in mg mL -1, of the original 1.00 mL crude extract if the absorbance acquired from the analysis is 0.200??
Example: A plant was discovered in a spring. To study the activity of the plant, 5.00g of the plant was finely grounded and mixed with an extraction buffer to make up a total of 1.00 mL crude plant extract. 19.00 ml of water was added to the plant extract. To determine the protein concentration of the extract, bovine serum albumin stock solution with a concentration of 200 μg mL -1 was used and mixtures with the following compositions and absorbances values were prepared (picture below). A 200 μg aliquot of the diluted plant extract was added with 300 μl of water and was subjected to Bradford assay. What is the protein concentration, in mg mL -1, of the original 1.00 mL crude extract if the absorbance acquired from the analysis is 0.200??
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Example: A plant was discovered in a spring. To study the activity of the plant, 5.00g of the plant was finely grounded and mixed with an extraction buffer to make up a total of 1.00 mL crude plant extract. 19.00 ml of water was added to the plant extract. To determine the protein concentration of the extract, bovine serum albumin stock solution with a concentration of 200 μg mL -1 was used and mixtures with the following compositions and absorbances values were prepared (picture below). A 200 μg aliquot of the diluted plant extract was added with 300 μl of water and was subjected to Bradford assay.
What is the protein concentration, in mg mL -1, of the original 1.00 mL crude extract if the absorbance acquired from the analysis is 0.200??
Transcribed Image Text:Tube #
Volume of BSA, mL
Volume of water, mL
Absorbance
0.00
2.00
0.000
0.40
1.60
0.094
13
0.60
1.20
0.175
120
0.80
0.282
160
0.40
p.342
2 00
0.00
0450
中
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