During cloning of eukaryotic genes, mRNA instead the original gene fragment is used to obtain the gene fragment to be cloned. The reason for this is that initial products of the eukaryotic genes contain introns and exons and is processed in cells to yield the final mature RNA. To obtain the sequence that will directly code the protein of interest, a DNA is produced using the mRNA as the template. This DNA is called______ and the enzyme is used to synthesize it is_______. a) cNDA and reverse transcriptase b) cDNA and restriction enzyme c) reverse DNA and reverse transcriptase d) reverse DNA and DNA ligase
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
18. During cloning of eukaryotic genes, mRNA instead the original gene fragment is used to obtain the gene fragment to be cloned. The reason for this is that initial products of the eukaryotic genes contain introns and exons and is processed in cells to yield the final mature RNA. To obtain the sequence that will directly code the protein of interest, a DNA is produced using the mRNA as the template. This DNA is called______ and the enzyme is used to synthesize it is_______.
a) cNDA and reverse transcriptase
b) cDNA and restriction enzyme
c) reverse DNA and reverse transcriptase
d) reverse DNA and DNA ligase
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