Bradford protein assay data: Absorbance Readings at 595nm You will need to graph this 0.104 for P1 data and determine the exact concentration of 0.098 for P2 protein (mg/ml) in samples (fractions) allowing for the dilution factor in the assay the 0.211 for P3 unknown 0.135 for P4 0.124 for P5 (0.2 into 1.0 ml = 1/5 dilution therefore multiply by 5). 0.95 for P6
Bradford protein assay data: Absorbance Readings at 595nm You will need to graph this 0.104 for P1 data and determine the exact concentration of 0.098 for P2 protein (mg/ml) in samples (fractions) allowing for the dilution factor in the assay the 0.211 for P3 unknown 0.135 for P4 0.124 for P5 (0.2 into 1.0 ml = 1/5 dilution therefore multiply by 5). 0.95 for P6
Chapter14: Formula Method
Section: Chapter Questions
Problem 5SST
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How would you create this graph
![Bradford protein assay data:
Absorbance Readings at 595nm You will need to graph this
0.104 for P1
data and
determine the
exact
concentration
of
0.098 for P2
protein (mg/ml) in
samples
(fractions) allowing for the
dilution factor in the assay
(0.2 into 1.0 ml = 1/5 dilution
therefore multiply by 5).
the
0.211 for P3
unknown
0.135 for P4
0.124 for P5
0.95 for P6](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F3271cffd-b89e-456d-ab0f-c7989200de69%2F09da020e-0517-410d-b739-d276333f1fb4%2Fph9fcm_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Bradford protein assay data:
Absorbance Readings at 595nm You will need to graph this
0.104 for P1
data and
determine the
exact
concentration
of
0.098 for P2
protein (mg/ml) in
samples
(fractions) allowing for the
dilution factor in the assay
(0.2 into 1.0 ml = 1/5 dilution
therefore multiply by 5).
the
0.211 for P3
unknown
0.135 for P4
0.124 for P5
0.95 for P6
![TUBE
P1
P2
P3
P4
P5
P6
P7
P8
P9
P10
P11
P12
BSA Standard (µg/ml)
Fraction (No)
10
20
30
40
T1
T2
T3
T4
T5
T6
Standard/BSA
stock solution
0.1
0.2
0.4
0.6
0.8
(ml)
Fraction (ml)
0.2
0.2
0.2
0.2
0.2
0.2
Distilled H20
0.8
0.7
0.6
0.4
0.2
0.6
0.6
0.6
0.6
0.6
0.6
(ml)
Dye reagent
0.2
0.2
0.2
0.2
0.2
0.2
0.2
0.2
0.2
0.2
0.2
0.2
(ml)](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F3271cffd-b89e-456d-ab0f-c7989200de69%2F09da020e-0517-410d-b739-d276333f1fb4%2Fynh5uda_processed.jpeg&w=3840&q=75)
Transcribed Image Text:TUBE
P1
P2
P3
P4
P5
P6
P7
P8
P9
P10
P11
P12
BSA Standard (µg/ml)
Fraction (No)
10
20
30
40
T1
T2
T3
T4
T5
T6
Standard/BSA
stock solution
0.1
0.2
0.4
0.6
0.8
(ml)
Fraction (ml)
0.2
0.2
0.2
0.2
0.2
0.2
Distilled H20
0.8
0.7
0.6
0.4
0.2
0.6
0.6
0.6
0.6
0.6
0.6
(ml)
Dye reagent
0.2
0.2
0.2
0.2
0.2
0.2
0.2
0.2
0.2
0.2
0.2
0.2
(ml)
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