Which of these CANNOT BE achieved using SDS-PAGE? I. Estimate the MW of a protein. II. Confirm the identity of a protein. III. Confirm the presence of quaternary structure in the protein. IV. Give a qualitative assessment of the protein extract purity. V. Determine the shape of the protein. a. II and V b. III and IV c. I, III and V d. I, II, and IV Which of the following statements are TRUE about the discontinuous gel in SDSPAGE? I. The sandwich effect occurs because of the lower mobility of the zwitterionic form of glycine compared to the protein band in the stacking gel. II. Because of the larger pores in the resolving gel, proteins move faster in the stacking gel, but decelerate at the boundary of the two gels. III. In the stacking gel, in terms of net negative charge of the species, the order is: zwitterionic glycine > proteins > chloride ion. IV. The higher pH in the resolving gel allows glycine to carry a more negative charge, resulting in higher mobilities compared to the proteins. V. The loading buffer that carries the sample supplies the glycine necessary for the sandwich effect. A. I and II B. I and IV C. II, III and V
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
pls explain why
Which of these CANNOT BE achieved using SDS-PAGE?
I. Estimate the MW of a protein.
II. Confirm the identity of a protein.
III. Confirm the presence of quaternary structure in the protein.
IV. Give a qualitative assessment of the protein extract purity.
V. Determine the shape of the protein.
a. II and V
b. III and IV
c. I, III and V
d. I, II, and IV
Which of the following statements are TRUE about the discontinuous gel in SDSPAGE?
I. The sandwich effect occurs because of the lower mobility of the zwitterionic
form of glycine compared to the protein band in the stacking gel.
II. Because of the larger pores in the resolving gel, proteins move faster in the
stacking gel, but decelerate at the boundary of the two gels.
III. In the stacking gel, in terms of net negative charge of the species, the order
is: zwitterionic glycine > proteins > chloride ion.
IV. The higher pH in the resolving gel allows glycine to carry a more negative
charge, resulting in higher mobilities compared to the proteins.
V. The loading buffer that carries the sample supplies the glycine necessary
for the sandwich effect.
A. I and II
B. I and IV
C. II, III and V
D. III, IV and V
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