1)Which methods can be used for total protein determination? Please explain briefly.  2) Evaluate the total protein determination methods according to their sensitivity. 3) Explain for what purposes the determination of total protein is used.

Q: Outline the steps to isolate a typical protein. What isachieved at each step?

A: Protein isolation is a process which involves isolation of proteins from a mixture of compounds.…

Q: Briefly explain the principle of Bradford Method of Total Protein Estimation

A: Asked : Principle of Bradford Method : Total Protein Estimation.

Q: Please provide in detail.

A: In liquid chromatography Elution is the process of separating or extracting material which get…

Q: Cemical analysis of a patient’s urine using a reagent strip gave negative protein and negative…

A: Urine reagent strips are used for qualitative analysis of abnormal conditions such as the presence…

Q: Evaluate total protein determination methods according to their sensitivity.

A: The total protein is determined in grams of protein per volume(in mL or dL) of the sample, that is…

Q: Explain the reason for using bile as a selective agent and the basis for the preliminary biochemical…

A: The amphipathic character of bile salts allows for their interaction with the lipid bilayer of cell,…

Q: Briefly describe the Kirby-Bauer test and its purpose.

A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…

Q: How can Biuret test be extended to quantitatively measure the concentration of protein

A: Proteins are the polymers of amino acids.

Q: What is the importance of the 6.25 multiplication factor for protein analysis (Kjeldahl methods)?

A: The Kjeldahl method for protein measurement was designed to be used for the measurement of nitrogen…

Q: How will you precipitate proteins from urine? Explain the principle behind your procedure. What are…

A: Urine is a product of metabolism produced through urea cycle. It is separated from blood through…

Q: What does an area of clearing indicate in biochemical test?

A: Measuring a nutrient or its metabolite in the blood, faeces, urine, or other tissues that have a…

Q: How does the ONPG/MUG test allow a sample to be assayed simultaneously for the presence of both…

A: The ONPG/MUG (4-methyl-umbelliferyl-beta-D-glucose/O-nitrophenyl-beta-D-galactopyranoside) test is a…

Q: What is this quantitative method called?

A: A mass- a spectrometry-based method that is used for quantifying and identifying changes in more…

Q: When assessing general analysis of protein concentration in food, absorbance at 280 nm vs…

A: Assessment of protein concentration in food is generally done by BCA assay or by Bradford method or…

Q: theraphy, What are the drawbacks of this technology? Enumerate at least 2and briefly explain ypur…

A: Gene therapy can be defined as the introduction of a new DNA into an old DNA to bring about genetic…

Q: Describe the two general types of protein microarrays. What aretheir possible applications?

A: A protein microarray is also known as the protein chip, is the high throughput method that is used…

Q: Chemical analysis of a patient’s urine using a reagent strip gave negative protein and negative…

A: Urinalysis is a diagnostic test performed over the urine sample collected from the patients. Results…

Q: I understand that microarrays are being used to define the molecular abnormality and the prognosis…

A: Leukemia and types

Q: What protein purification methods can be used to ensure that the resulting protein is soluble ?

A: The method used for protein purification that can be used to ensure that the resulting protein is…

Q: Search for the principles and the uses of the following methods for protein measurements and provide…

A: The different techniques used in protein separation are electrophoresis, dialysis, and…

Q: How do you determine protein concentration using bicinchoninic acid assay?

A: Proteins are polymers that are composed of amino acids. Amino acids are in turn made up of a central…

Q: Immunofluorescence and Fluorescent live cell imaging techniques can both be used to determine…

A: Immunofluorescence is a technique in which fluorochrome labeled-antibodies are used to detect the…

Q: Explain the protein determination methods and when to use which method?

A: Protein quantitation is an important process and is very much essential and a necessary step to be…

Q: What other approaches can be used to determine protein concentration?

A: Introduction :  Proteins are nitrogenous organic substances made up of one or more elongated chains…

Q: hat is an ONPG test? WHat is he principle behind this assay.

A: Fermentation of lactose happens by bacteria which depends on two enzymes - permease (allows lactose…

Q: Explain the extraction method and the procedure for extraction of Echinolone by using…

A: Chromatography works on the principle of molecules in a mixture being applied to a surface or a…

Q: Protein Analysis.

A: Standard volume of BSA (ml)  Equiv. conc. (mg/ml) Absorbance  at 550nm 1. 0.10 0.6 0.062 2. 0.20…

Q: How is tight quality control for accurate protein production achieved? Why is it important? thank…

A: Introduction:   The endoplasmic reticulum (ER) functions as a protein-folding factory, with…

Q: Quantitatively describe the unique focusing capability of IEF and identify factors that increase…

A: IEF stands for isoelectric focusing which is a technique used to resolve a mixture of proteins on a…

Q: Explain why a semi-log plot should be used for determining antigen concentration by ELISA.

A: ELISA(enzyme linked immnosorbant assay)is a qualitative and quantitative approach that is followed…

Q: Find a method that uses some form of HPLC for the analysis of proteins. What was the stationary…

A: Protein analysis by chromatography is a widely used technique for separating, identifying, and…

Q: Increasing the saturation of the ammonium sulfate is a prerequisite in isolating a  target protein…

A: Since you have asked multiple questions, we will solve the first question for you. If you want a…

Q: Which fractions measured from your gel filtration experiment had the most and least amount of…

A: Gel filtration chromatography or size exclusion chromatography is a technique used to separate…

Q: What are some common protein identification techniques?

A: Proteome: The set of all expressed proteins in a cell, tissue, or organism. Proteomics: A science…

Q: A bioactive linear peptide was detected in amphibian toxıns. The peptide was found to be made up of…

A: Given; the amphibian toxin has only 10 amino acids out of which 2 are cysteine (nonpolar)and 2 are…

Q: Name the process of separation and purification of expressed protein before marketing.

A: Introduction  Biotechnology is the branch of biology which deals with the various techniques to…

Q: why are lysozyme and ovoalbumin the recommended standards used in the Bradford protein assay and why…

A: Lysozyme is an enzyme found in tears, saliva and mucus which plays a role in destroying bacterial…

Q: Identify the most commonly used technique for diagnosing COVID-19? And the clinical sample for each…

A: Coronavirus disease or COVID 19 is an infectious disease caused by a new strain of coronavirus. CO'…

Q: What are the biochemical test used to assay fatty acids and cholesterol?

A: Fatty acids are carboxylic acid derivative of aliphatic chain hydrocarbon. It can have long or short…

Q: (3) What is a protein database? Give examples (and links) of some protein database

A: Bioinformatics is described as the application of tools of computation as well as the analysis to…

Q: Problem 5(classwork): a.)explain why certain sedative medicines which alter breathing can produce…

A: Sedatives are medications that are involved in lowering brain activity. They are used to relax the…