What does -log(p-value) tell in immunoprecipitation mass spectrometry? What does log2 Fold change say? Generally, what does the graph of immunoprecipitation mass spectrometry tell (ie. how to read its graph)?

Q: Why should the cell density of the inoculum be as small as possible? Why can we not standardize the…

A: The process of introduction of microbes into a nutrient media or other similar environments which…

Q: Report the slope and the y-intercept below.  Slope () =                    =     y-intercept () =…

A: In the given graph, an equation is provided. y=-4E-09x + 0.4021R2= 0.1691 The graph represents the…

Q: 1)Which methods can be used for total protein determination? Please explain briefly.  2) Evaluate…

A: Plasma is the fluid part of the blood which consists of water, proteins, electrolytes, metabolites…

Q: What is the importance of the 6.25 multiplication factor for protein analysis (Kjeldahl methods)?

A: The Kjeldahl method for protein measurement was designed to be used for the measurement of nitrogen…

Q: Why should there be 3 banding techniques (G, Q, and R)? Aside from their differences in appearance…

A: Banding techniques are utilized to understand the staining effects in the chromosomes so that the…

Q: 4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes…

A: Calibration graphs are used to estimate the concentration of unknown sample by plotting it against…

Q: When the gel is removed from the chamber in SDS-PAGE, only the “pre-stained” protein standard is…

A: SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis), is commonly used as a method…

Q: What is this quantitative method called?

A: A mass- a spectrometry-based method that is used for quantifying and identifying changes in more…

Q: If you increase the sample size (n) of a sample, will the following values increase, decrease,…

A: Answer :  1. When n increases, the standard error of the sample mean will decrease. This is due to…

Q: Fluorescence confocal microscopy (FCM) - STK38 monoclonal antibody (M01), clone 2G8- 1F3. 200 μm a.…

A: The STK38 Monoclonal Antibody from LifeSpan BioSciences is a Mouse Monoclonal antibody. This…

Q: Analysis of a 49kDA protein is aimed with a western blot technique. For this purpose, "whole cell…

A: Western blot is a technique in which the concentration of a protein is determined using SDS PAGE…

Q: For the following sequence, what is the approximate annealing temperature? 5'-AGCTACGATCAGGTCA-3'

A: PCR (Polymerase Chain Reaction) is divided into three main steps : Denaturation of DNA, Annealing of…

Q: The following list of proteins are found in a mixture and are to be separated by 2D gel…

A: In 2D gel electrophoresis, is a 2 step-based separation of protein.  In the first dimension, the…

Q: Explain the purpose of the Kirby-Bauer diffusion test?

A: Kirby-Bauer diffusion test/Disk diffusion method It is most widely used antibiotic susceptibility…

Q: In

A: STR typing or short tendum repeat analysis is a molecular biology technique used to study or analyze…

Q: S-CCT CCT C The protein assay kit that we will use to quantitate horseradish peroxidase requires…

A: Protien assay - It is the process of determining the amount or concentration of a specific protein…

Q: Describe a general scheme for protein purification of a protein like hemoglobin

A: Protein Purification: The protein Purification is done is to retain more number of function proteins…

Q: You have an extract of BL21(DE3) cells containing a soluble, 75 kDa recombinant protein that has a…

A: Chromatography is an is a technique used to separate or purify compounds from the mixture of…

Q: Proteins secreted by bacteria can be used as biomarkers for diagnostics. Give a concise account of…

A: The identification of protein antigens from pathogens can serve as a diagnostic tool. Several…

Q: The

A: To calculate the amount of the 2000x ampicillin stock to add to your 1.0-L culture to achieve a…

Q: In the Hershey–Chase experiment, the radioactive label 32P was present inside bacterial cells (i.e.,…

A: DNA is an organic molecule that transmits information from generation to generation. Several…

Q: Streptococci are divided into different groups called serotypes. Consult this or other textbooks and…

A: Streptococci are divided into many groups called serotypes based on the Lancefield classification…

Q: Using a schematic diagram, summarize the following steps in preparing competent cells for…

A: prepare competent cells for transformation ;- Add 20 µL of each ligation reaction or 1 ng of uncut…

Q: A student conducted an experiment on protein isolation from mungbean. After isolating the protein,…

A: Bradford assay is an example for a general protein assay. It is used to quantify the unknown amount…

Q: A plaque assay is performed beginning with 1 mL of a solution containing bacteriophages. This…

A: Concentration is the term used to define the amount of a substance present in the solution or the…

Q: In urea assay (Catalog #K375-100) what is the nmol (range) it can detect?

A: Urea assay (catalogue #K375-100) is a colorimetric assay used to quantify the urea in any given…

Q: What is the DNA concentration (ug/ml) of an undiluted sample with A260 reading without background of…

A: Pure DNA molecule is extracted by measuring the absorbance of DNA molecule at 260 nm. Pure DNA shows…

Q: Please answer both asap If there were only 2 tests for COVID-19 and Test A has a high sensitivity…

A: In general, high sensitivity tests have low specificity. In other words, they are good for catching…

Q: The GC content of the DNA in the genome of a newly discovered bacterium is 46%, what is the…

A: Chargaff’s rules state that the DNA content from any cell of any organism should always have a 1:1…

Q: 1) In MALDI-TOF data, when performing peptide mass mapping search, a) The number of peptides…

A: Protein identification with the help of mass spectrometry (MS) is an major step in proteomic…

Q: A packing plant fills bags with cement. The weight X kg of a bag of cement can be modelled by a…

A: Introduction A Binder Is A Substance Used In Construction That Sets, Hardens, And Clings To Other…

Q: Electrophoresis 1.)a.) A protein required 6.8 min to travel 82 cm to the detector in a 96 cm -long…

A: Electrophoresis describes the separation of charged particles and their migration in the influence…

Q: Which fractions measured from your gel filtration experiment had the most and least amount of…

A: Gel filtration chromatography or size exclusion chromatography is a technique used to separate…

Q: w

A: In NGS, GC-poor or GC-rich sequences, lead to an uneven coverage or even no coverage of reads across…

Q: (3) What is a protein database? Give examples (and links) of some protein database

A: Bioinformatics is described as the application of tools of computation as well as the analysis to…

Q: What are the reaction bat the molecular level of denaturation of frying egg. Explain step by step…

A: Egg is a good dietary source of protein. Egg also contain mono and polyunsaturated fatty acids,…

Q: At higher amounts of protein, the Bradford assay is not linear. Consider the plot to the right: what…

A: Bradford assay is used to determine the unknown concentration of proteinbin a sample from standard…

Q: Which technique is used to identify an unknown protein ?

A: Proteomics is the study of protein analysis. In this study the comparison, of proteins behaviours…

Q: a sample was found to contain 10.0ug/L of nitrate. a spike of 5 ug/L was added to a replicate…

A: Introduction  Most nitrate is utilised as fertiliser for lawns or crops. Additionally, nitrate and…