When performing a separation by ion-exchange chromatography, the rate of elution of an amino acid is influenced by three main factors: a. Temperature b. Solution (buffer) pH c. Ionic strength (buffer concentration) of the eluent  Briefly, explain how each of these parameters influences elution rate.    Please provide only typed answer solution no handwritten solution needed allowed

Biochemistry
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Chapter1: Biochemistry: An Evolving Science
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When performing a separation by ion-exchange chromatography, the rate of elution of an amino acid is influenced by three main factors: a. Temperature b. Solution (buffer) pH c. Ionic strength (buffer concentration) of the eluent 

Briefly, explain how each of these parameters influences elution rate. 

 

Please provide only typed answer solution no handwritten solution needed allowed 

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