QUESTION 2 The Bradford method described in the Background section was used to determine protein concentrations of known and unknown samples The following results was obtained: Table 1: Absorbance at 595nm obtained for different concentrations of protein standards using the Bradford method 0.50 Protein (mg/ml) 0.75 1.50 0.00 0.125 0.25 1.00 2.00 Unknown Abs. BSA 0.130 0.350 0.620 0.870 1.120 1.400 1.600 1.105 Abs. Blg 0.080 0.230 0.460 0.520 0.410 0.510 | 0.700 1.8 1.7 1.6 1.5 Abs. BSA Abs. Blg 1.4 1.3 1.2 11 1.0 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0.0 0.00 0.25 0.50 0.75 1.00 1.25 1.50 1.75 2.00 Protein concentration (mg/ml) Absorbancy (595nm)
QUESTION 2 The Bradford method described in the Background section was used to determine protein concentrations of known and unknown samples The following results was obtained: Table 1: Absorbance at 595nm obtained for different concentrations of protein standards using the Bradford method 0.50 Protein (mg/ml) 0.75 1.50 0.00 0.125 0.25 1.00 2.00 Unknown Abs. BSA 0.130 0.350 0.620 0.870 1.120 1.400 1.600 1.105 Abs. Blg 0.080 0.230 0.460 0.520 0.410 0.510 | 0.700 1.8 1.7 1.6 1.5 Abs. BSA Abs. Blg 1.4 1.3 1.2 11 1.0 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0.0 0.00 0.25 0.50 0.75 1.00 1.25 1.50 1.75 2.00 Protein concentration (mg/ml) Absorbancy (595nm)
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
Transcribed Image Text:QUESTION 2
The Bradford method described in the Background section was used to determine protein concentrations of known and unknown samples
The following results was obtained:
Table 1: Absorbance at 595nm obtained for different concentrations of protein standards using the Bradford method
Protein (mg/ml)
0.00
0.125
0.25
0.50
0.75
1.00
1.50
2.00
Unknown
Abs. BSA
0.130
0.350
0.620
0.870
1.120 1.400
1.600
1.105
Abs. Blg
0.080
0.230
0.460
0.520
0.410 | 0.510
0.700
1.8
1.7
1.6
1.5
Abs. BSA
Abs. Blg
1.4
1.3
1.2
1.1
1.0
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0.0
0.00
0.25
0.50
0.75
1.00
1.25
1.50
1.75
2.00
Protein concentration (mg/ml)
Absorbancy (595nm)
Transcribed Image Text:Calculate the concentration of the unknown protein in mg/ml.
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 2 steps
Recommended textbooks for you
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON