QUESTION 5 You plan to run a standard curve of BSA to determine the concentration of protein in an unknown sample. You begin with a stock solution of BSA, 2.5 mg/mL, and require a final volume of 500 μL. Select the most appropriate response to complete the dilution table shown below. Table. Volume of BSA stock and water required to prepare BSA standards. BSA concentration in standard Vol. of Stock (µL) Vol. of water (µL) (ug/mL) 0 0 500 100 20 480 250 50 450 700 1200 1500 1700 240 300 340 260 200 160 Vol. of Stock 40 μ L, Vol. of water 460 μL O Vol. of Stock 14000 μ L, Vol. of water 3.6 mL O Vol. of Stock 140 μL, Vol. of water 360 μL O Vol. of Stock 0.0025 mL, Vol. of water 500 μL O Vol. of Stock 1400 μ L, Vol. of water 3600 μL

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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QUESTION 5
You plan to run a standard curve of BSA to determine the concentration of protein in an unknown sample. You begin with a stock solution of BSA, 2.5 mg/mL, and
require a final volume of 500 μL. Select the most appropriate response to complete the dilution table shown below.
Table. Volume of BSA stock and water required to prepare BSA standards.
BSA concentration
in standard
Vol. of Stock
(µL)
Vol. of water
(µL)
(ug/mL)
0
0
500
100
20
480
250
50
450
700
1200
1500
1700
240
300
340
260
200
160
Vol. of Stock 40 μ L, Vol. of water 460 μL
O Vol. of Stock 14000 μ L, Vol. of water 3.6 mL
O Vol. of Stock 140 μL, Vol. of water 360 μL
O Vol. of Stock 0.0025 mL, Vol. of water 500 μL
O Vol. of Stock 1400 μ L, Vol. of water 3600 μL
Transcribed Image Text:QUESTION 5 You plan to run a standard curve of BSA to determine the concentration of protein in an unknown sample. You begin with a stock solution of BSA, 2.5 mg/mL, and require a final volume of 500 μL. Select the most appropriate response to complete the dilution table shown below. Table. Volume of BSA stock and water required to prepare BSA standards. BSA concentration in standard Vol. of Stock (µL) Vol. of water (µL) (ug/mL) 0 0 500 100 20 480 250 50 450 700 1200 1500 1700 240 300 340 260 200 160 Vol. of Stock 40 μ L, Vol. of water 460 μL O Vol. of Stock 14000 μ L, Vol. of water 3.6 mL O Vol. of Stock 140 μL, Vol. of water 360 μL O Vol. of Stock 0.0025 mL, Vol. of water 500 μL O Vol. of Stock 1400 μ L, Vol. of water 3600 μL
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