3. Incubate the solution at room temperature for 5 min.4.Read the absorbance at 595nm of the blank and test solutions.Calculate the protein concentration (in mg/ml) of an unknown sample with an absorbancy value of 0.996 using the standard curve below.1.51.41.31.21.11.00.90.80.70.60.50.40.30.20.10.00.00.10.20.30.40.50.60.70.80.91.01.11.21.31.41.51.6BSA concentration (mg/ml)wuseS Áɔuequosqy QUESTION 3An unknown protein was determined using the following method:Bradford methodReagentsStock Bradford reagent: Dissolve 100 mg Coomassie Brilliant Blue G250 in a mixture consisting of 100ml of 85% phosphoric acid, 50ml of 95% ethanol and 50ml 1M NaOH.Store at 4°C until precipitation occurs, at which point it is discarded.Working Bradford reagent: Prepare fresh by diluting 10ml of stock Bradford reagent to 250ml with distilled water.Stock bovine serum albumin (BSA) solution (10mg/ml): Dissolve 0.2g BSA in 20ml distilled water.Working BSA concentration range: 0.5 – 1.25 mg/mlMethod1. Prepare the following test tubes:Table 1: Preparation of test tubes for the Bradford methodBlankTube 1Tube 2Tube 3Tube 4Tube 5UnknownDistilled H201.0 ml0.9ml0.9ml0.9ml0.9ml0.9ml0.8ml0.25 mg/ml BSA0.1 ml0.5 mg/ml BSA0.1 ml0.75 mg/ml BSA0.1 ml1.0 mg/ml BSA0.1 ml1.25 mg/ml BSA0.1mlUnknown0.2mlproteinWorking4.0ml4.0ml4.0ml4.0ml4.0ml4.0ml4.0mlBradford2.After each addition of the working Bradford reagent, thoroughly mix the resultant solution.

Bradford method of protein estimation is quick and simple method of protein estimation using…

Answered: Calculate the protein concentration (in…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

A dosage of 3 mcg/kg/min has been ordered for an 87.4 kg adult. The solution has a strength of 50 mg in 250 mL. Calculate the dosage per min.
Make a standard curve by using the values given in the table and determine the concentration of unknown sample? Tube 0,5 µg/ µl BSA 0,15 M NaCl Comassi Brillant Blue Solution Absorbance 595 nm 1 0 100 µl 900 µl 0 2 10 µl 90 µl 900 µl 0,105 3 20 µl 80 µl 900 µl 0,233 4 30 µl 70 µl 900 µl 0,41 5 40 µl 60 µl 900 µl 0,455 6 50 µl 50 µl 900 µl 0,589 Unknown Sample 10 µl 90 µl 900 µl 1,2
Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.192 and A650=0.090, taking into account the dilution factor (100), and the total volume of extract (45). Note your answer to 2 decimal places.
What volume of a 40% v/v solution must be used to produce 500mL of 5% v/v solution?
1.0 ml of serum albumin (BSA) solution was precisely diluted to 100 ml with a buffer solution, and the absorbance at 280 nm was measured from this buffer solution at a distance of 1 cm from the light. The result was A=1.0. BSA is 0.1%(=0.1g/l). The absorptivity is e(0.1%)=0.667, so 1g/l solution gives an absorbance of 0.667. What was the original protein content? Report in the result unit mg/ml with an accuracy of 0.1 mg.
Calculate the 5 different volumes of BSA needed for the standard curve. Prepare a table showing the volume of BSA and water needed in the cuvette for each concnetration
How many liters of a 7 M NaOH stock solution would you need to make 780 mL of a 153 mM NaOH dilution? (m.w. = 40.00 g/mol)
The concentration of the conjugate base in 100 mL of 50 mM CH3COOH/CH3COO- (pKa = 4.76) at pH 5.00 is ______mM. If 100 μL of 1.0 M NaOH is added, the buffer pH is expected to shift to ______ please answer it and explain .
Norepinephrine solution is available in 4 mL ampoules containing 1 mg of norepinephrine per mL. The solution must be diluted prior to administration. If a physician orders a 1.6 mg% (w/v) dilution in sodium chloride injection, how many mL of the sodium chloride injection must be added to the contents of one 4-mL ampule? Round your answer to the nearest whole milliliter.
Using the table above, make the following unit conversions. Determine this by moving the decimal point the appropriate number of places. 1. 25 μL mL 8. 8500 µL = 2. 3 L = µL 9. 64 TL = GL 3. 150 cL = dL 10. 120000 nL = µL 4. 5000 pL = nL 5. 75 L = kL 6. 800 nL = cL 7. 6 hL = ML In addition to being able to convert among different units of measurement, geneticists also need to
If you had a cell suspension of 2.5 x 10^4 cells/mL and plated 100uL into each well (4 wells), how many cells were added per well?
Calculate the percentage of stated content in paracetamol tablets using the calbration curve given above and the following data: Data • Weight of 20 tablets¼12.2243 g • Weight of tablet powder taken¼152.5 mg • Stated content per tablet¼500 mg • Initial extraction volume¼200 ml. Dilution steps • 20 ml into 100 ml • 10 ml into 100 ml • Area of chromatographic peak for paracetamol extracted from the tablets¼44 519.

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