Question 5We can determine the copper(II) ion content of a sample using spectrophotometry.The following data was collected. Construct a calibration plot (x - [Cu2+], Y-Absorbance)and determine the slope.Absorbance[Cu2+], M0.0652.00E-060.2056.00E-060.3381.00E-050.4741.40E-050.5981.80E-053.3 x 10-23.3 x 1043.1 x 10-52.3 x 10-3

Let,x−axisrepresentamountofCu2+.Let,y−axisrepresentabsorbance. Using given table as data points, we…

Answered: Question 5 We can determine the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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QUESTION 1 What is 105 ng ml in: a. ug/ml b. mg 1 C. ug1 d. g/ul (Please list each answer on a separate line, beginning with the corresponding letter. Don't forget units for each answer!) For the toolbar, press ALT+F10 (PC) or ALT-FN+F10 (Mac). 14px E E YA BIUS Paragraph Arial 田 Bヨ田田用国田EEt o
QUESTION 3 You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0.225 1 0.310 0.420 3 0.510 4 0.610 0.720 0.810 7 0.915 8 0.950 9 0.980 10 0.990 Set up a standard curve. What would the quality of the line-fit (R2 value) be if you exclude experimental outliers? (Give you answer to 4 decimal places)
Question 33 Orciprenaline (C₁₁H₁7NO3, 211.258 g/mol) is a bronchodilator used in the treatment of asthma. The BP assay for orciprenaline tablets is described below. HO The following information was obtained during the assay: Weight of 20 tablets = 2.5564 g Weight of tablet powder assayed = 0.5266 g Absorbance reading - 0.5868 Stated content per tablet = 20 mg A (1%, 1 cm) at 276 nm = 72.3 OH H N OH "Weigh and powder 20 tablets. Shake a quantity of the powder containing 80 mg of orciprenaline sulphate with 50 mL of 0.01 M hydrochloric acid (HCI), filter and add sufficient 0.01 M HCI to the filtrate to produce 100 mL. Dilute 10 mL to 100 mL with 0.01 M HCI and measure the absorbance of the resulting solution at the maximum at 276 nm." Orciprenaline 4 Answer the following questions, explaining your reasoning clearly. a) Calculate the amount of orciprenaline in the tablet powder analyzed. b) Calculate the average amount of orciprenaline per tablet. c) Calculate the percentage of the stated…
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Question 2 You are checking a calculation that a foundation pharmacy student has performed. The question asks that 120 suppositories weighing 1 gram are to be made, containing 300mg Zinc Oxide. The displacement value of ZnO is 4.7 in the base that is to be used. How many grams of base would be required? units - g Answer to 2 decimal places Amount of base required = theoretical amount – displaced amount Where; Displaced amount = (amount of drug in g/DV of drug).
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QUESTION 2 You obtained the following raw data when setting up a Biuret standard curve: Absorbancy BSA (mg/ml) 540nm 0.158 1 0.210 2 0.260 0.305 4 0.360 0.410 0.455 7 0.510 8 0.530 9. 0.550 10 0.554 After blanking against a biuret-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.324 was obtained. curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / Set up a standard ml (up to 3 significant figures). 5
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Question 4. New December 18, 2020. The nitrite in a series of standard solutions (mg/L, n = 5) are converted to azo dye and the slope of the calibration curve is 2.0 ppm. A 10.00-mL mineral water sample is treated in the same way as standards and diluted to a final volume of 100.00-mL. It gives an absorbance of 0.80. The absorbance of blank solution under the same conditions is 0.10. Calculate ppm (mg/L) of NO2 (46 g/mol) and the molarity of NaNO2 (69 g/mol) in the original sample B I Ss II !!
QUESTION 4 You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0.225 1 0.310 0.420 0.510 4 0.610 0.720 6 0.810 7 0.915 8 0.950 9. 0.980 10 0.990 Set up a standard curve. What would the quality of the line-fit (R2 value) be if you do not exclude experimental outliers? (Give you answer to 4 decimal places) N M
QUESTION 2 You obtained the following raw data when setting up a Bradford standard curve: Absorbancy BSA (mg/ml) 595nm 0.225 0.310 2 0.420 0.510 4 0.610 0.720 0.810 0.915 0.950 9 0.980 10 0.990 After blanking against a bradford-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.583 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).

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