Homework Help is Here – Start Your Trial Now!
Science
Biology
Molecular weight Chemical 66.5 kDa Bovine serum albumin (BSA) 180 Glucose (C6H1206) 58.4 Sodium chloride (NaCl) Sodium citrate (Na3C6H507) 258 Sodium dodecyl sulfate (SDS, NaC 12 H2 5SO4) 288 Tris (C4H11NO3) 121.1 1. Calculate how much glucose you need to make 500 ml of a 5% glucose solution. 2. Calculate the molarity of the glucose solution you made in Q1. 3. How would you make 2 liters of a 0.5 M Tris buffer, pH 7.6? 4. How would you make 500 ml of 5 M NaCI? 5. How would you make 1 liter of 2 M sodium citrate? 6. How would you make 1 liter of stock 20X SSC (standard sodium citrate) buffer that is 3 M NaCl, 0.3 sodium citrate, using the solutions you made in Q4 and Q5 above? 7. Calculate how much SDS you need to make 800 ml of a 5% SDS solution. 8. Calculate how much SDS and SSC you need from the solutions made in Q6 and Q7 to make 1 liter of 0.1X SSC, 0.1% SDS. 9. How would you make 1 liter of 20 mM Tris, 10 mM NaCl from the solutions made in Q3 and Q4? 10. How much BSA would you need to add to the solution in Q9 to make it 1% BSA? 131% XPO W DOC MacBook Pro DII DD F6 F 7 F 8 F9 F10 F11 & 7 +

Molecular weight Chemical 66.5 kDa Bovine serum albumin (BSA) 180 Glucose (C6H1206) 58.4 Sodium chloride (NaCl) Sodium citrate (Na3C6H507) 258 Sodium dodecyl sulfate (SDS, NaC 12 H2 5SO4) 288 Tris (C4H11NO3) 121.1 1. Calculate how much glucose you need to make 500 ml of a 5% glucose solution. 2. Calculate the molarity of the glucose solution you made in Q1. 3. How would you make 2 liters of a 0.5 M Tris buffer, pH 7.6? 4. How would you make 500 ml of 5 M NaCI? 5. How would you make 1 liter of 2 M sodium citrate? 6. How would you make 1 liter of stock 20X SSC (standard sodium citrate) buffer that is 3 M NaCl, 0.3 sodium citrate, using the solutions you made in Q4 and Q5 above? 7. Calculate how much SDS you need to make 800 ml of a 5% SDS solution. 8. Calculate how much SDS and SSC you need from the solutions made in Q6 and Q7 to make 1 liter of 0.1X SSC, 0.1% SDS. 9. How would you make 1 liter of 20 mM Tris, 10 mM NaCl from the solutions made in Q3 and Q4? 10. How much BSA would you need to add to the solution in Q9 to make it 1% BSA? 131% XPO W DOC MacBook Pro DII DD F6 F 7 F 8 F9 F10 F11 & 7 +

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question

Can you show me how to do question 6?

Molecular weight Chemical 66.5 kDa Bovine serum albumin (BSA) 180 Glucose (C6H1206) 58.4 Sodium chloride (NaCl) Sodium citrate (Na3C6H507) 258 Sodium dodecyl sulfate (SDS, NaC 12 H2 5SO4) 288 Tris (C4H11NO3) 121.1 1. Calculate how much glucose you need to make 500 ml of a 5% glucose solution. 2. Calculate the molarity of the glucose solution you made in Q1. 3. How would you make 2 liters of a 0.5 M Tris buffer, pH 7.6? 4. How would you make 500 ml of 5 M NaCI? 5. How would you make 1 liter of 2 M sodium citrate? 6. How would you make 1 liter of stock 20X SSC (standard sodium citrate) buffer that is 3 M NaCl, 0.3 sodium citrate, using the solutions you made in Q4 and Q5 above? 7. Calculate how much SDS you need to make 800 ml of a 5% SDS solution. 8. Calculate how much SDS and SSC you need from the solutions made in Q6 and Q7 to make 1 liter of 0.1X SSC, 0.1% SDS. 9. How would you make 1 liter of 20 mM Tris, 10 mM NaCl from the solutions made in Q3 and Q4? 10. How much BSA would you need to add to the solution in Q9 to make it 1% BSA? 131% XPO W DOC MacBook Pro DII DD F6 F 7 F 8 F9 F10 F11 & 7 +
Transcribed Image Text:Molecular weight Chemical 66.5 kDa Bovine serum albumin (BSA) 180 Glucose (C6H1206) 58.4 Sodium chloride (NaCl) Sodium citrate (Na3C6H507) 258 Sodium dodecyl sulfate (SDS, NaC 12 H2 5SO4) 288 Tris (C4H11NO3) 121.1 1. Calculate how much glucose you need to make 500 ml of a 5% glucose solution. 2. Calculate the molarity of the glucose solution you made in Q1. 3. How would you make 2 liters of a 0.5 M Tris buffer, pH 7.6? 4. How would you make 500 ml of 5 M NaCI? 5. How would you make 1 liter of 2 M sodium citrate? 6. How would you make 1 liter of stock 20X SSC (standard sodium citrate) buffer that is 3 M NaCl, 0.3 sodium citrate, using the solutions you made in Q4 and Q5 above? 7. Calculate how much SDS you need to make 800 ml of a 5% SDS solution. 8. Calculate how much SDS and SSC you need from the solutions made in Q6 and Q7 to make 1 liter of 0.1X SSC, 0.1% SDS. 9. How would you make 1 liter of 20 mM Tris, 10 mM NaCl from the solutions made in Q3 and Q4? 10. How much BSA would you need to add to the solution in Q9 to make it 1% BSA? 131% XPO W DOC MacBook Pro DII DD F6 F 7 F 8 F9 F10 F11 & 7 +
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 4 steps with 3 images

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Violence
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS