Molecular weight Chemical 66.5 kDa Bovine serum albumin (BSA) 180 Glucose (C6H1206) 58.4 Sodium chloride (NaCl) Sodium citrate (Na3C6H507) 258 Sodium dodecyl sulfate (SDS, NaC 12 H2 5SO4) 288 Tris (C4H11NO3) 121.1 1. Calculate how much glucose you need to make 500 ml of a 5% glucose solution. 2. Calculate the molarity of the glucose solution you made in Q1. 3. How would you make 2 liters of a 0.5 M Tris buffer, pH 7.6? 4. How would you make 500 ml of 5 M NaCI? 5. How would you make 1 liter of 2 M sodium citrate? 6. How would you make 1 liter of stock 20X SSC (standard sodium citrate) buffer that is 3 M NaCl, 0.3 sodium citrate, using the solutions you made in Q4 and Q5 above? 7. Calculate how much SDS you need to make 800 ml of a 5% SDS solution. 8. Calculate how much SDS and SSC you need from the solutions made in Q6 and Q7 to make 1 liter of 0.1X SSC, 0.1% SDS. 9. How would you make 1 liter of 20 mM Tris, 10 mM NaCl from the solutions made in Q3 and Q4? 10. How much BSA would you need to add to the solution in Q9 to make it 1% BSA? 131% XPO W DOC MacBook Pro DII DD F6 F 7 F 8 F9 F10 F11 & 7 +

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Violence

Violence is defined as a physical act that causes or is intended to cause harm. Violence can cause physical, emotional, or both types of harm. Violence is defined by the World Health Organization as the intended use of physical force and power that causes threat or harm against oneself, another person, or against a community or group, and has the potential to cause damage, death, mental distress, maldevelopment, or poverty.