Choose among proteins A, B, C, and D. 1. Protein that will most strongly bind to an anion exchange colum.  2. Protein that will elute last in gel filtration chromatography.  3. Protein that will elute first in hydrophobic interaction chromatography.  4. Protein that will elute last in a carbohydrate containing colum.

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter24: Lipid Biosynthesis
Section: Chapter Questions
Problem 23P
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Choose among proteins A, B, C, and D.

1. Protein that will most strongly bind to an anion exchange colum. 
2. Protein that will elute last in gel filtration chromatography. 
3. Protein that will elute first in hydrophobic interaction chromatography. 
4. Protein that will elute last in a carbohydrate containing colum. 

Consider a mixture comprised of the following proteins below:
MW Charge
Affinity to a
Protein
%Polarity
(kDa) (pH 7.4) specific ligand
A
760
-3
None
45
B
300
+1
Carbohydrate
70
C
80
-1
None
60
55
+2
None
25
Transcribed Image Text:Consider a mixture comprised of the following proteins below: MW Charge Affinity to a Protein %Polarity (kDa) (pH 7.4) specific ligand A 760 -3 None 45 B 300 +1 Carbohydrate 70 C 80 -1 None 60 55 +2 None 25
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