A certain heterotetrameric protein was run in PAGE under different reducing and denaturing conditions. The resulting block diagram is shown below: How many bands, and at what MWs, would appear in a separate SDS-PAGE run, with dithiothreitol + HCl included in the loading buffer? A. 2 : 150, 200 kDa B. 2 : 100, 250 kDa C. 3 : 50, 100, 150 kDa D. 4 : 50, 100, 150, 350 kDa
A certain heterotetrameric protein was run in PAGE under different reducing and denaturing conditions. The resulting block diagram is shown below: How many bands, and at what MWs, would appear in a separate SDS-PAGE run, with dithiothreitol + HCl included in the loading buffer? A. 2 : 150, 200 kDa B. 2 : 100, 250 kDa C. 3 : 50, 100, 150 kDa D. 4 : 50, 100, 150, 350 kDa
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
A certain heterotetrameric protein was run in PAGE under different reducing and
denaturing conditions. The resulting block diagram is shown below:
How many bands, and at what MWs, would appear in a separate SDS-PAGE run,
with dithiothreitol + HCl included in the loading buffer?
A. 2 : 150, 200 kDa
B. 2 : 100, 250 kDa
C. 3 : 50, 100, 150 kDa
D. 4 : 50, 100, 150, 350 kDa
Transcribed Image Text:150
S-S
S-S
50
50
h-bond
h-bond
loo
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