Bradford Assay Fill in the average A595 and A595 of sample minus A595 of blank. What to do? Calculating the average absorbance of the standards Subtracting the absorbance of the blank (the sample with 0 g/ml) from the absorbance of the other samples Concentration (micrograms/ml) A595 - trial 1 A595 - trial 2 A595 - trial 3 average A595 A595 of sample minus A595 of blank 0 1.501 1.446 1.447 2 1.624 1.558 1.559 5 1.731 1.749 1.712 10 1.901 1.838 1.892 15 2.161 2.108 2.228 18 2.231 2.277 2.319
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
Bradford Assay
Fill in the average A595 and A595 of sample minus A595 of blank.
What to do?
- Calculating the average absorbance of the standards
- Subtracting the absorbance of the blank (the sample with 0 g/ml) from the absorbance of the other samples
|
Concentration (micrograms/ml) |
A595 - trial 1 |
A595 - trial 2 |
A595 - trial 3 |
average A595 |
A595 of sample minus A595 of blank |
|
0 |
1.501 |
1.446 |
1.447 |
||
|
2 |
1.624 |
1.558 |
1.559 |
||
|
5 |
1.731 |
1.749 |
1.712 |
||
|
10 |
1.901 |
1.838 |
1.892 |
||
|
15 |
2.161 |
2.108 |
2.228 |
||
|
18 |
2.231 |
2.277 |
2.319 |
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