Q. You have now carried out the Somogyi Nelson determination of reducing sugars and measured the absorption spectra of Tube 9. What is the optimum wavelength you should use to monitor the Somogyi Nelson Assay why? Table 1._SOMOGYI-NELSON DTERMENATION OF REDUCING SUGARS Tube 1 2 3 4 5 6 7 8 9 10 D-Glucose (160 µg/mL), mL 0 0 0.125 0.125 0.25 0.25 0.375 0.375 0.5 0.5 H2O, mL 0.5 0.5 0.375 0.375 0.25 0.25 0.125 0.125 0 0 D-Galactose (160 µg/mL), mL - - - - - - - - - - D-Arabinose (160 µg/mL), mL - - - - - - - - - - Glucose-Galactose Mixture, mL - - - - - - - - - - Absorbance (710 nm) 0.049 0.051 0.138 0.151 0.271 0.26 0.382 0.395 0.454 0.444 Absorbance - blank 0 0 0.088 0.101 0.221 0.21 0.332 0.345 0.404 0.394 Final [Reducing sugar] (µg/mL) 0 0 40 40 80 80 120 120 160 160 Tube 11 12 13 14 15 16 D-Glucose (160 µg/mL), mL - - - - - - H2O, mL - - - - - - D-Galactose (160 µg/mL), mL 0.5 0.5 - - - - D-Arabinose (160 µg/mL), mL - - 0.5 0.5 - - Glucose-Galactose Mixture, mL - - - - 0.5 0.5 Absorbance (710 nm) 0.461 0.468 0.426 0.416 0.321 0.312 Absorbance - blank 0.411 0.418 0.376 0.366 0.271 0.262 Final [Reducing sugar] (µg/mL) - - - - - -

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Related questions

Concept explainers

Question

Q. You have now carried out the Somogyi Nelson determination of reducing sugars and measured the absorption spectra of Tube 9. What is the optimum wavelength you should use to monitor the Somogyi Nelson Assay why?

Table 1._SOMOGYI-NELSON DTERMENATION OF REDUCING SUGARS

Tube	1	2	3	4	5	6	7	8	9	10
D-Glucose (160 µg/mL), mL	0	0	0.125	0.125	0.25	0.25	0.375	0.375	0.5	0.5
H₂O, mL	0.5	0.5	0.375	0.375	0.25	0.25	0.125	0.125	0	0
D-Galactose (160 µg/mL), mL	-	-	-	-	-	-	-	-	-	-
D-Arabinose (160 µg/mL), mL	-	-	-	-	-	-	-	-	-	-
Glucose-Galactose Mixture, mL	-	-	-	-	-	-	-	-	-	-
Absorbance (710 nm)	0.049	0.051	0.138	0.151	0.271	0.26	0.382	0.395	0.454	0.444
Absorbance - blank	0	0	0.088	0.101	0.221	0.21	0.332	0.345	0.404	0.394
Final [Reducing sugar] (µg/mL)	0	0	40	40	80	80	120	120	160	160

Tube	11	12	13	14	15	16
D-Glucose (160 µg/mL), mL	-	-	-	-	-	-
H₂O, mL	-	-	-	-	-	-
D-Galactose (160 µg/mL), mL	0.5	0.5	-	-	-	-
D-Arabinose (160 µg/mL), mL	-	-	0.5	0.5	-	-
Glucose-Galactose Mixture, mL	-	-	-	-	0.5	0.5
Absorbance (710 nm)	0.461	0.468	0.426	0.416	0.321	0.312
Absorbance - blank	0.411	0.418	0.376	0.366	0.271	0.262
Final [Reducing sugar] (µg/mL)	-	-	-	-	-	-

Table 1._SOMOGYI-NELSON DTERMENATION OF REDUCING SUGARS
Tube
1
2
3
4
7
8
9
10
D-Glucose (160 µg/mL), mL
0.125
0.125
0.25
0.25
0.375
0.375
0.5
0.5
H20, mL
0.5
0.5
0.375
0.375
0.25
0.25
0.125
0.125
D-Galactose (160 ug/mL),
mL
D-Arabinose (160 ug/mL),
mL
Glucose-Galactose Mixture,
mL
Absorbance (710 nm)
0.049
0.051
0.138
0.151
0.271
0.26
0.382
0.395
0.454
0.444
Absorbance - blank
0.088
0.101
0.221
0.21
0.332
0.345
0.404
0.394
Final [Reducing sugar]
40
40
80
80
120
120
(ug/mL)
160
160
Tube
11
12
13
14
15
16
D-Glucose (160 µg/mL), mL
H20, mL
D-Galactose (160 µg/mL), mL
0.5
0.5
D-Arabinose (160 µg/mL), mL
0.5
0.5
Glucose-Galactose Mixture, mL
0.5
0.5
Absorbance (710 nm)
0.461
0.468
0.426
0.416
0.321
0.312
Absorbance - blank
0.411
0.418
0.376
0.366
0.271
0.262
Final [Reducing sugar] (µg/mL)

Expert Solution

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.

Similar questions